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Hao
Shen
Associate Professor,
Dept of Microbiology
Microbiology,
Virology and Parasitology Program
Address
303D Johnson Pavilion
3610 Hamilton Walk
Philadelphia, PA 19104-9999
Office tel.: 215 573-5259
Lab tel.: 215 573-2890
Fax: 215 573-9068
E-mail:hshen@mail.med.upenn.edu
Link(s)
Dr.
Shen's Microbiology Faculty Page
Education
Jiangxi University: BS (Microbiology), 1983.
University of California-Riverside: PhD (Molecular Genetics), 1992.
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Research
Interests
- Immune surveillance of intracelluar and emerging
infectious agents.
Key words: Listeria, Ebola, T lymphocyte,
bacterial pathogenesis, vaccine.
Search PubMed for articles
Description
of Research
We are interested in understanding how the microbial
virulence strategies affect the nature and magnitude of the
host response and how the resulting immune response influences
the course of infection. We use the intracellular bacterium
Listeria monocytogenes, a category B agent, as a model and
employ a multidisciplinary approach that combines recent technical
and conceptual advances in molecular genetics, microbial pathogenesis,
cell biology and immunology. By genetically manipulation of
the bacterium, we are identifying different aspects of bacterial
antigens that influence their ability to induce a T cell response
and to serve as a protective target. We are also combining
our ability to genetically manipulate the bacterium with the
use of knock-out mice to examine how various immune effectors
counter different microbial virulence factors and to identify
new immune correlates of protection.
We are also applying the knowledge gained from
our basic research and our expertise in both microbial pathogenesis
and cellular immunology to study emerging pathogens such as
Ebola virus and B. anthracis. We are identifying T cell epitopes
in Ebola and examining how the soluble glycoprotein (sGP)
of Ebola may interfere with the ability of dendritic cells
to prime T cell responses. We are investigating the role of
phospholipases and hemolysin in mediating escape of B. anthracis
from phagosome of macrophages and are studying the early immune
response to the pulmonary form of anthrax infection using
various KO murine models. The long-term objective of our studies
is to provide a platform for rational design of effective
vaccines to combat today’s complex and evolving diseases.
Recent
Publications
Shedlock DJ, Shen H. Requirement for CD4 T cell help in generating
functional CD8 T cell memory. Science 2003 Apr 11;300(5617):337-9.
Zenewicz LA, Skinner JA, Goldfine H, Shen H. Listeria monocytogenes
Virulence Proteins Induce Surface Expression of Fas Ligand
on T lymphocytes. Mol Microbiol 2004 Mar;51(5):1483-92.
Pearce EL, Shedlock DJ, Shen H. Functional Characterization
of MHC Class II-Restricted CD8+CD4- and CD8-CD4- T Cell Responses
to Infection in CD4-/- Mice. J Immunol. 2004 Aug
15;173(4):2494-9.
Lau L, Jiang J, Shen H. In vivo modulation of the T cell
response and protective immunity by TCR antagonism. J
Immunol. 2005, 174: 7970-76.
Zenewicz LA, Wei Z, Goldfine H, Shen H. Phosphatidylinositol-specific
phospholipase of Bacillus anthracis downmodulates the immune
response. J Immunol. 2005, 174: 8011-16.
Lab
Rotation
Projects for 2006-2007
- Modulation of immune response by bacterial
virulence factors
- Molecular basis of functional T cell memory
- Lab
personnel:
Alison Crawford, PhD
Connie Krawczyk, PhD
Luana Atherly, PhD
Joanna DiSpirito
Erika Pearce
John Northrop
Amy Troy
last updated 7/2005
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