Cell and Developmental Biology Microscopy Core

Laser-Scanning Confocal Microscopes

These microscopes are ideal for imaging fixed or live thick (10 — 500 microns) fluorescent specimens.

Leica TCS SP8 Confocal

Room 01-126D Smilow

Leica TCS SP8 confocalFeatures

  • DMI 6000 inverted microscope
  • LAS X software for acquisition
  • Excitation laser lines: 405, 488, 552, 638 nm
  • Three spectral detection channels: 2 PMTs and 1 HyD detector
  • Tokai-Hit stagetop incubation chamber
  • FRAP and FRET wizards
  • Motorized x-y stage for and tiling applications

Best suited for: confocal imaging of fixed or live samples, including those requiring incubation; FRAP, photoactivation, or photoconversion in live cells or organisms; FRET

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Leica STED 3X Super-resolution Microscope

Room B30A Anatomy-Chemistry

FeaturesLeica SP8 STED super-resolution microscope

  • Two depletion lasers (592 and 660 nm) for super-resolution imaging of green and red dyes
  • Leica DMI 6000 inverted microscope
  • Pulsed white-light laser for tunable excitation from 470 to 670 nm
  • CW laser lines for conventional confocal imaging: 405, 458, 488, 514 nm
  • High-speed resonant scanner for live-cell imaging
  • 3 HyD and 2 PMT detectors
  • Tokai Hit stagetop incubator
  • Motorized xy stage
  • LAS X software for acquisition; Huygens Professional software for deconvolution

Best suited for: ultra-high resolution (< 50 nm lateral, < 200 nm axial) imaging of fixed & live samples; fast confocal imaging of live specimens.

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Leica TCS SP8 Multiphoton Confocal

Room 514A CRB

Features

Visible laser lines (all diode or DPSS lasers): 405, 488, 514, 552, 638
Coherent Chameleon Vision II + MPX wavelength extender for tunable excitation from 740 — 1300 nm
Leica DM 6000 upright microscope
High-speed resonant scanner for live-cell imaging
2 HyD and 3 PMT  confocal detectors
HyD and PMT dual NDDs for reflected light (fluorescence or SHG)
dual PMT NDD for transmitted light (SHG)
25x, 1.00 NA HC IRAPO motCORR objective for multiphoton imaging
Motorized xy stage
LAS X software for acquisition

Best suited for: standard confocal imaging of samples suited to upright platform; multiphoton imaging of thick tissue; second-harmonic generation imaging of collagen

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Zeiss LSM 710 Confocal

Room 01-126C Smilow

Features

  • Zeiss AxioObserver inverted microscope
  • ZEN 2012 software for acquisition
  • Excitation laser lines: 405, 458, 488, 514, 561, 594, 633 nm
  • Three spectral detection channels, including a 32-channel detector
  • Environmental chamber and motorized x-y stage for live imaging and tiling applications

Best suited for: confocal imaging of fixed or live samples, including those requiring incubation; FRAP, photoactivation, or photoconversion in live cells or organisms; FRET; spectral characterization and linear unmixing of unorthodox dye combinations

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Zeiss LSM 880

Room 1175A BRB

Features

  • Zeiss AxioObserver inverted microscope
  • ZEN v2.3 software for acquisition
  • Excitation laser lines: 405, 458, 488, 514, 561, 633 nm
  • Three spectral detection channels: 2 metal alkali PMT + 1 GaAsP detector
  • Airyscan module with FAST option for enhanced resolution, speed, and sensitivity
  • Environmental chamber and motorized x-y stage for live imaging and tiling applications

Best suited for: confocal imaging of fixed or live samples, including those requiring incubation; FRAP, photoactivation, or photoconversion in live cells or organisms; Airyscan module provides 1.7x increase in resolution; FAST module provides 4x increase in speed.

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