Cell and Developmental Biology Microscopy Core

Spinning-Disk Confocals

These systems are optimized for rapid confocal imaging of live specimens.

Spinning Disk Confocal #1

Room 1175B BRB II/III

Features

  • Olympus IX71 inverted microscope
  • Hamamatsu ImagEM EMCCD camera
  • Excitation laser lines: 442, 488, 523, 561 nm (for CFP, GFP, YFP, and RFP)
  • Motorized xy stage for multi-point acquisition
  • Andor MicroPoint ablation laser (405 or 435 nm) for ablation or FRAP
  • LCI Chamlide stagetop incubation system for live mammalian cell imaging
  • MetaMorph acquisition software

Best suited for: fast confocal imaging of sensitive, live samples; FRAP in live cells or organisms; FRET sensors (CFP-YFP or GFP-RFP)

Close


Spinning Disk Confocal #2

Room 1175D BRB II/III

Features:

  • Olympus IX81 inverted microscope
  • Excitation laser lines: 405, 488, 561 (for Hoechst, GFP, RFP)
  • Andor iXon3 EMCCD camera
  • Motorized x-y stage for multi-point acquisition or tile scanning
  • Roper iLas2 ablation laser (405 nm) for FRAP or photoactivation
  • Okolab stagetop incubation chamber
  • MetaMorph acquisition software

Best suited for: fast confocal imaging of sensitive, live samples; FRAP, photoactivation, photoconversion in live cells or organisms

Close


© The Trustees of the University of Pennsylvania | Site best viewed in a supported browser. | Site Design: PMACS Web Team.