UPenn Cell & Developmental Biology
Equipment Details
Deltavision Deconvolution Microscope
Zeiss LSM 710 Confocal
Location: 1041A BRB II/III
List of Features:
- Inverted microscope accommodates standard microscope slides, Lab-tek II chambered coversglasses, 35 mm glass-bottomed dishes or glass-bottomed multi-well plates.
- Zeiss ZEN 2010 software for acquisition
- Excitation laser lines: 405, 458, 488, 514, 561, 594, 633
- Adjustable spectral detection, including 32-channel detector
- Environmental chamber and motorized x-y stage for live imaging applications
F. M. Kirby Center Zeiss LSM 510 NLO/META
Location: B-110 Richards Building
List of Features :
- Inverted microscope accommodates standard microscope slides, Lab-tek II chambered coversglasses, or 35 mm glass-bottomed dishes
- Zeiss AIM software for acquisition
- Excitation laser lines: 458, 488, 514, 543, 633 PLUS tunable Ti-Sapphire laser for multi-photon excitation of DAPI and other dyes.
Spinning Disk Confocals 1 & 2
Location: 1076C & 1074C BRB II/III
List of Features:
- Inverted microscope accommodates standard microscope slides, Lab-tek II chambered coversglasses, or 35 mm glass-bottomed dishes
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Suitable fluorophores:
- GFP & RFP or CFP & YFP (spinning disk 1)
- Hoechst, GFP, & RFP (spinning disk 2)
- Cameras:
- Hamamatsu ImagEM EMCCD camera OR Photometrics HQ2 14-bit high-resolution CCD camera (spinning disk 1)
- Andor iXon3 EMCCD camera (spinning disk 2)
- Motorized x-y stage for multi-point acquisition
- Computer-controlled laser module for photoactivation or FRAP experiments (both systems)
- Stagetop environmental chamber (both systems)
- MetaMorph acquisition software (both systems)
Leica TCS SP2 Confocal
Location: 941A BRB II/III
List of Features:
- Upright microscope accommodates standard microscope slides or 35-mm dishes
- Leica LCS software for acquisition
- Excitation laser lines: 458, 488, 514, 561, 633.
- Adjustable spectral detection on all channels
- NOTE: this confocal cannot image DAPI or any other violet-excited dyes
Deltavision Deconvolution Station
Location: 1074B BRB II/III
List of Features:
- Inverted microscope accommodates slides, 35-mm glass-bottomed dishes, or Lab-Tek II chambered coverglasses.
- Photometrics CoolSnap HQ high-resolution CCD camera
- Applied Precision x-y-z precision motorized stage for multi-point acquisition of image stacks
- Software-controlled filter wheels with DAPI, FITC, TRITC, Cy5, CFP, YFP filter sets; phase contrast & DIC available
- Environmental chamber surrounding entire microscope for temperature and CO2 control
- Deltavision Softworx software for acquisition and processing.
Leica Widefield Microscope
Location: 941A BRB II/III
List of Features:
- Leica DMRBE upright microscope with halogen and mercury arc lamps
- QImaging MicroPublisher 5.0 MP high resolution color CCD camera
- iVision for Mac acquisition software
- phase contrast, DIC, and polarized light transmitted light options
- Filter sets for standard 3-color fluorescence imaging (DAPI, FITC, TRITC)
Zeiss Widefield Microscope
Location: B-110 Richards
List of Features:
- Zeiss Axioplan upright microscope with Zeiss FluoArc mercury lamp
- Zeiss AxioCam HRm high-resolution monochrome CCD camera
- AxioVision 4.5 software for acquisition and analysis
- Filter sets for standard 4-color imaging (DAPI, FITC, TRITC, Cy5); also CFP and YFP filter sets
Philips XL 20 Scanning Electron Microscope
Location: 1076B BRB II/III
Features:
Polaron Au/Pd sputter coater, Denton critical point dryer
Image Processing Computers
Location: 1005 BRB II/III
Use of the workstations in room 1005 is on a first-come, first-served basis.
iMac Core Workstation (Mac OS 10.6.x)
27-inch iMac with 3.1 GHz Quad-Core Intel i5 processor + AMD Radeon HD graphics processor: perfect for those large multidimensional data sets!
- Volocity 5, sophisiticated software for visualization, deconvolution, and quantitative analysis of 3- and 4-dimensional image data. (Mac and Windows)
- Fiji (Fiji Is Just ImageJ)
G5 Mac Workstation (Mac OS 10.6.x)
- Volocity 5, sophisiticated software for visualization, deconvolution, and quantitative analysis of 3- and 4-dimensional image data. (Mac and Windows)
- Fiji (Fiji Is Just ImageJ)
Windows 7 Workstation
- Volocity 5
- MetaMorph Premier (powerful quantitative analysis software for 2D/3D image sets. Accommodates most file formats. Contact Andrea to be added to the user list.)
- Fiji (Fiji Is Just ImageJ)
- GIMP (open-source substitute for Adobe Photoshop & Illustrator)
- OpenOffice (substitute for Microsoft Office)
Windows XP Workstation
- Leica LCS Simulator (fully function version of LCS for display, processing, and analysis of Leica confocal images)
- MetaMorph Basic (MetaMorph Premier without specialized application modules)
- Volocity 5
- Zeiss AxiovisionLE, v. 4.6
- ImageJ bundle
- Adobe Suite
Deltavision Workstation (Linux)
Full version of SoftWoRx, Deltavision's processing and deconvolution software, used primarily for post-acquisition processing of images acquired on the Deltavision Widefield imaging station.