Cell & Developmental Biology

UPenn Cell & Developmental Biology

Equipment Details

Anatomy-Chemistry Suite B30

Zeiss LSM 510 NLO/META

FeaturesZeiss LSM 510

Best suited for: confocal imaging of fixed fluorescent samples or live samples at room temperature; second-harmonic generation imaging of collagen in unfixed or fixed tissue.

Zeiss Widefield Microscope

Features

Best suited for: widefield imaging of fixed, fluorescent samples mounted on slides with coverslips

BRB II/III Suite 1175 (11th floor BRB II/III)

Zeiss LSM 710 ConfocalZeiss LSM 710

Features

Best suited for: confocal imaging of fixed or live samples, including those requiring incubation; FRAP, photoactivation, or photoconversion in live cells or organisms; FRET; spectral characterization and linear unmixing of unorthodox dye combinations

Spinning Disk Confocal 1Spinning Disk Confocal 1

Features

Best suited for: fast confocal imaging of sensitive, live samples; FRAP in live cells or organisms; FRET sensors (CFP-YFP or GFP-RFP)

Deltavision Deconvolution MicroscopeDeltavision

Features

Best suited for: high resolution widefield deconvolution of thin (< 15 microns) fluorescent samples; long time-lapse imaging using transmitted or epifluorescence.

BRB Leica Widefield MicroscopeBRB Leica Widefield

Features

Best suited for: Color imaging of fixed tissue stained with chromogenic dyes; polarized light microscopy; simple immunofluorescence imaging of fixed samples

SCTR Suite 01-126, Room 01-127 (Smilow Translational Research Center, first floor)

Leica TCS SP8 ConfocalLeica TCS SP8

Features

Best suited for: confocal imaging of fixed or live samples, including those requiring incubation; FRAP, photoactivation, or photoconversion in live cells or organisms; FRET


Spinning Disk Confocal 2 Spinning Disk Confocal 2

Features:

Best suited for: fast confocal imaging of sensitive, live samples; FRAP, photoactivation, photoconversion in live cells or organisms

SCTR Leica Widefield Microscope

Features:

Best suited for: high-resolution widefield deconvolution microscopy of thin (<15 microns), fixed samples; widefield epifluorescence imaging of fixed samples.

EVOS FL Auto Cell Imaging System

List of Features: EVOS

Best suited for: Single images or tile scans of fixed tissue stained with chromogenic dyes or fixed cells stained with fluorescent markers; live imaging of cells or organisms; hypoxia experiments

In addition to the above acquisition systems, the CDB Microscopy Core also provides access to off-line image analysis workstations.

Currently the Core supports the following software:

 

 

Dept. of Cell & Developmental Biology
1157 BRB II/III
421 Curie Boulevard
Philadelphia, PA 19104-6058
Tel: (215) 573-9306
Fax: (215) 898-9871