UPenn Cell & Developmental Biology
Equipment Details
New! Zeiss 2-photon
F. M. Kirby Center Zeiss 2-photon Confocal
Location: B-110 Richards Building
Best suited for: multiple QDot detection; imaging on thick tissue sections or whole mount (100-300 microns); live imaging on specimens at room temperature; spectral characterization
List of Features :
Zeiss Axiovert 200M inverted microscope
Lasers: Coherent Chameleon fs pulsed NIR laser (720-970 nm); Argon (458, 488, 514); 543 nm HeNe, 633 nm HeNe 
META detector for spectral imaging
Two regular confocal detectors, three non-descanned detectors, one transmission detector
Can rotate scan field, do xzy scans, FRAP
Accommodates standard microscope slides with coverslips sealed to the slide or 35 mm, glass-bottomed (Matek) Petri dishes.
Windows 2000 operating system.
Spinning Disk Confocal
Location: 1076C BRB II/III
Best suited for: Multi-point time lapse imaging of living cells or thicker organisms that are susceptible to photodamage or that need environmental control.
List of Features:
Olympus IX71 inverted microscope
CSU10 Yokogawa scan head
Lasers: 442 nm, 488 nm, 523 nm, 561 nm (all diode)
Choice of Hamamatsu ImagEM 16-bit cooled EMCCD camera or Photometrics HQ2 14-bit cooled CCD camera
MicroPoint Galvo laser module for photoactivation or FRAP experiments
Pathology Devices stagetop incubator w/ objective heater for temperature and CO2 control
MetaMorph acquisition software, Windows XP operating system
Leica Confocal
Location: 941A BRB II/III
Best suited for: Acquisition of 3D fluorescence stacks on thick (10 - 150 micron), bright, non-bleaching samples mounted on a standard microscope slide with a coverslip.
List of Features:
Leica DM 6000 upright microscope
Lasers: Argon laser (458, 488, 514); 561 nm diode; 633 nm HeNe
Spectral imaging capability, rotating scan head, FRAP, time lapse, etc.
Up to 5 channels of acquisition (4 fluorescence + 1 transmission)
Windows XP operating system
Zeiss Confocal
Location: 1041A BRB II/III
Best suited for: Acquisition of 3D fluorescence stacks on thick (10- 150 micron), bright, non-bleaching samples or FRAP experiments on living cells.
List of Features:
Zeiss Axiovert 100 inverted microscope
Lasers: Coherent Enterprise UV laser (358, 364 nm); Argon (458, 488, 514); 543 nm HeNe, 633 nm HeNe
Up to 5 channels of acquisition (4 fluorescence + 1 transmission)
Can rotate scan field, do xzy scans, FRAP
Accommodates standard microscope slides with coverslips sealed to the slide or 35 mm, glass-bottomed (Matek) Petri dishes.
Windows 2000 operating system.
Deltavision Deconvolution Station
Location: 1074B BRB II/III
Best suited for: Multi-point time-lapse imaging using transmitted light or fluorescence for samples requiring environmental control; stack acquisition of wide-field fluorescence images for deconvolution (samples should be less than 20 microns thick)
List of Features:
Olympus IX70 inverted microscope with 300 W xenon lamp illumination
Photometrics CoolSnap HQ 12-bit CCD camera
Applied Precision x-y-z precision motorized stage for multi-point acquisition of image stacks
Software-controlled filter wheels with DAPI, FITC, TRITC, Cy5, CFP, YFP filter sets; phase contrast & DIC available
4X, 10X, 20X, 40X dry objectives; 60X & 100X oil objectives
Environmental chamber for temperature and CO2 control
Deltavision Softworx software for acquisition and processing. Processing options include constrained iterative deconvolution using measured or theoretical point-spread functions, nearest-neighbor deconvolution, various filters, image arithmetic.
Linux operating system
Zeiss Widefield Microscope
Location: 1141A BRB II/III
Best suited for: Widefield fluorescence imaging of fixed samples
List of Features:
Zeiss Axioplan upright microscope with Zeiss FluoArc mercury lamp
Zeiss AxioCam HRm high-resolution monochrome CCD camera
2.5x, 10x, 20x dry objectives, 40X oil immersion objective
Filter sets for standard 4-color imaging: DAPI, FITC, TRITC, Cy5; also CFP and YFP filter sets
AxioVision 4.5 software for acquisition and analysis
Philips XL 20 Scanning Electron Microscope
Location: 1076B BRB II/III
Features:
Polaron Au/Pd sputter coater, Denton critical point dryer
Image Processing Computers
Location: 1005 BRB II/III
Use of the workstations in room 1005 is on a first-come, first-served basis.
Volocity Workstation (Mac OS X or Windows XP)
Mac Pro workstation with dual Intel processors can run either Mac OS or Windows XP natively. Login instructions are located next to the computer.
- Volocity 4, sophisiticated software for visualization, deconvolution, and quantitative analysis of 3- and 4-dimensional image data. (Mac and Windows)
- MBF ImageJ bundle
FOCAL2 Workstation (Windows XP)
Access is restricted to registered users only. Contact Andrea or Yuri if you would like to be added to the user group.
- Full version of Zeiss AIM software (for display, processing and analysis of LSM images)
- Zeiss Image Browser (for display, minor editing of LSM images)
- LCS Lite (for display, minor editing of Leica confocal images)
- MetaMorph Premier (powerful quantitative analysis software for 2D/3D image sets. Accommodates most file formats. Contact Andrea to be added to the user list.)
- MBF ImageJ bundle
- Adobe Suite
Leica Workstation (Windows XP)
Access is restricted to registered users only. Contact Andrea or Yuri if you would like to be added to the user group.
- Leica LCS Simulator (fully function version of LCS for display, processing, and analysis of Leica confocal images)
- MetaMorph Basic (MetaMorph Premier without specialized application modules)
- IPLab for Windows
- Zeiss AxiovisionLE, v. 4.6
- MBF ImageJ bundle
- Adobe Suite
Deltavision Workstation (Linux)
Access is restricted to registered users only. Contact Yuri if you would like to use this computer.
Full version of SoftWoRx, Deltavision's processing and deconvolution software, used primarily for post-acquisition processing of images acquired on the Deltavision Widefield imaging station.