UPenn Cell & Developmental Biology

CDB Zebrafish Core

The CDB Zebrafish Core facility provides services and training to Penn faculty interested in using the zebrafish model system as part of their research program.

Services provided by the core:

• Newly fertilized embryos for microinjection of antisense morpholinos or cDNAs.
• Embryos and larvae for RNA in situ hybridization experiments, histological and immunohistochemical analyses, or experiments that require genomic DNA, total RNA or protein.
• Fixed embryos for RNA in situ hybridization, histological or immunohistochemical analyses
• Embryos and larvae derived from transgenic or mutant lines.
• Maintenance of transgenic and mutant lines.
• An introduction to the zebrafish system that includes hands on training with microinjection and molecular analyses.

Costs:

• Clutch of 100 newly fertilized wild type, mutant or transgenic embryos - $40 for CDB faculty; $60 for non-CDB faculty
• Fixed embryos suitable for RNA in situ hybridization, histological or immunohistochemical analyses (clutch of 50) - $75 for CDB faculty, $100 for non-CDB faculty.
• Maintenance of transgenic or mutant lines - $1,000 per year
• Registration fee (includes introduction to zebrafish system with hands-on training) - $500 per user.

Request for Core Services :

• Please submit a completed Zebrafish Core Request Form.
• To access the core, Principal Investigators must provide a fund number for billing and provide written or email confirmation of core procedures.

Contacts:

Michael Pack - Core Director

Jie He - Core Technician