Nonhuman Primate Core Services
- Nonhuman Primate Pilot Grant Program
- Regulatory oversight and assistance in acquiring nonhuman primates
- Consultation on study design for nonhuman primate studies
- Routine veterinary care and husbandry
- Perform aspects of HIV-related research studies using nonhuman primates
- Clinical Laboratory Services
- Anatomic Pathology
- Confocal microscopy and image analysis
- Flow cytometry and immunology
Flow cytometric analysis is performed in the TNPRC Flow Cytometry laboratory, which offers both flow cytometric analysis and cell sorting. The TNPRC Flow Cytometry facility includes a Becton Dickinson FACSVerse, a BD LSRFortessa, a BDFACSAria I, and a BD FACSArray. It also has a computer station dedicated for flow cytometry analysis equipped with FlowJo Software. The FACSVerse is a fully automated instrument capable of 10 parameter analysis and is adaptable to sampling from tubes or plates. It is equipped with a flow sensor for volumetric measurements, thereby providing cell counts as well as the capability to determine absolute counts of stained populations. The LSRFortessa is a 4-laser system capable of 15 parameter analysis. The FACSAria I is a high-speed cell sorter capable of analysis of up to 14 parameters and live cell sorting of up to 4 simultaneous populations. The FACSAria I is housed under BSL-3 conditions allowing live cell sorting of macaque cells. These instruments (primarily the FACSVerse and LSRFortessa) are used for routine polychromatic immunophenotypic analysis of cell populations. These instruments will be used for most analyses including enumeration of CD4+ and CD8+ T cells and subsets, and analysis of antigen specific immune responses using intracellular cytokine responses.These are routine techniques in our laboratory.
In addition we have an AutoMACS automated magnetic cell sorter, capable of sorting through very large numbers of cells (up to 4 x 109 cells) in just a few minutes, to obtain as many as 2 x 108 target cells isolated from the larger population. The combined use in sequence of the AutoMACS to rapidly enrich rare cells from a very large starting population, followed by sorting with the flow cytometer to identify and sort cells by complex multi-parameter criteria, allows for the isolation of useful numbers of rare cells characterized in fine detail from large cell populations.
For all of these assays the core can provide full service including data analysis or more limited service depending on investigator needs and expertise.
- Viral characterization, isolation and production
Core Services Contact:
Andrew A. Lackner, D.V.M., Ph.D.