Nonhuman Primate Core Services
- Nonhuman Primate Pilot Grant Program
- Regulatory oversight and assistance in acquiring nonhuman primates
- Consultation on study design for nonhuman primate studies
- Routine veterinary care and husbandry
- Perform aspects of HIV-related research studies using nonhuman primates
- Clinical Laboratory Services
- Anatomic Pathology
- Confocal microscopy and image analysis
Confocal microscopy services for multilabel in situ immunophenotyping are provided by the Confocal Microscopy and Image Analysis lab at the TNPRC. This lab, within the Division of Comparative Pathology, provides assistance and training with confocal microscopy, immunofluorescent staining, image analysis, and digital imaging. The lab utilizes a Leica TCS SP8 five- channel confocal microscopy imaging system with a DMI-6000 inverted microscope and six strong laser lines that allows routine 4 and 5 color imaging plus differential interference contrast. The confocal system offers benefits such as: a) Multi-dimensional imaging, as it is possible to obtain images in four dimensions, length (x axis), width (y axis), depth (z axis), and time (t); b) Resolution improvement; c) Contrast improvement; and d) Multicolor imaging.
The Image analysis system for transmitted light is based on a Leica DMRE microscope, a Spot camera and ImagePro software on a dedicated work station. This system allows for computer assisted analysis and quantitation of image features such as numbers of positive cells, etc.
- Flow cytometry and immunology
- Viral characterization, isolation and production
Core Services Contact:
Andrew A. Lackner, D.V.M., Ph.D.