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Penn Center for AIDS Research

Viral / Molecular Core High Density Sequencing Support

Core Services Main Page | Virology | Molecular | Sequencing | SGA Services | Intergrated vDNA | Other

High density sequencing is an emerging technology with the capacity to generate millions of sequences, with up to a billion bases of sequence in a single run. This approach is ideal for characterizing complex populations such as HIV and SIV, HBV/HCV, and sites of retroviral DNA integration.

The Viral/Molecular Core is now offering Illumina sequencing as a service. In each case, amplicons will be generated from samples of interest, then sample sequences determined. Sequences are subject to stringent quality control algorithim and provided to users in fasta format. Bioinformatic analysis is available on a fee-for-service basis. The core also provides advice on experimental design, sequence work up, statistical analysis, and long term data storage.

Several services are offered.

  1. Initial consultation on experimental design (free of charge)
  2. Deep sequencing of viral genomes
  3. Sequencing of sites of retroviral DNA integration
  4. Programmer time for data analysis

Pricing

The Core provides support for each of these areas through the expertise of Dr. Frederic Bushman (Core Co-Director). Advanced bioanalytic support and analysis is offered collaboratively through the Biostatistics and Data Management Core (Core G) by Dr. Hongzhe Li (Core G Co-Investigator).

For further information on utilizing V/M Core support for high density sequencing, contact Aoife Doto.

Ordering Information:
Order High Density Sequencing services through the Path BioResource at Penn. Instructions for the Path BioResource can be found here on the CFAR website or on the Path BioResource site itself.

Core Service Contact:
Aoife Doto
Viral and Molecular Core
Email: rochea@mail.med.upenn.edu

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Pricing

Service CFAR Academic List Unit
Kapa $20.00 $24.20 $36.20 sample
Pico Green $5.00 $6.05 $9.05 sample
TapeStation $15.00 $18.15 $27.15 sample
miSeq-300 $1,785.00 $2,159.85 $3,230.85 lane
miSeq-500 $2,040.00 $2,468.40 $3,692.40 lane
miSeq-micro-300 $1,465.00 $1,772.65 $2,651.65 lane
miSeq-nano-300 $1,135.00 $1,373.35 $2,054.35 lane
miSeq-nano-500 $1,270.00 $1,536.70 $2,298.70 lane
miSeq-v3_150 $1,615.00 $1,954.15 $2,293.15 lane
miSeq-v3_600 $2,370.00 $2,867.70 $4,489.70 lane
Hour-Lab $115.00 $139.15 $208.15 hour
Analysis $205.00 $248.05 $371.05 instance
Sample prep (purify DNA, amplify) $22.00 $26.62 $39.82 per sample
Preparation for samples for integration site analysis $249.00 $301.29 $451.94 per sample
Integration site work up, gene therapy $619.63 $749.75 $1,124.63 per sample
Programmer Time $90.00 $108.90 $162.35 per hour
Bioinformatics Analyst Time $150.00 $181.50 $271.50 per hour

Penn CFAR Member pricing is available to faculty and lab personnel at the University of Pennsylvania, Children's Hospital of Philadelphia, and the Wistar Institute.

For more information and to request services, click here!

Contact Aoife Doto for further information at rochea@mail.med.upenn.edu