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Penn Center for AIDS Research

Viral / Molecular Core Virology Services

Core Services Main Page | Virology | Molecular | Sequencing | SGA Services | Intergrated vDNA | Other

Ordering Information:
Order Virology Support services through the Path BioResource at Penn. Instructions for the Path BioResource can be found here on the CFAR website or on the Path BioResource site itself.

Core Service Contact:
Farida Shaheen, Ph.D.
Technical Director, Viral and Molecular Core
Tel: 215-898-5180
Fax: 215-573-4446
Email: farida@mail.med.upenn.edu

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Virus isolation from clinical samples

The Core will isolate HIV-1 from plasma, PBMC, fluids, or tissues provided by CFAR investigators (under approval of his/her human subjects IRB approval, if appropriate). Isolation can be done using either standard PBMC coculture methods or high stringency CD8-depleted cells. Cultures are typicaly maintained for up to 6 weeks.

Isolates will be provided as a tissue culture supernatant seed stock. Stocks are quantified by p24 antigen content and confirmed mycoplasma-free before distribution.

Ordering Information:
Order Virology Support services through the Path BioResource at Penn. Instructions for the Path BioResource can be found here on the CFAR website or on the Path BioResource site itself.

Fees:

Item

CFAR

Virus Isolation - PBMC or plasma

$250.00

Virus isolation - tissue or other source

$275.00

Virus isolation/high stringency (4 weeks)

$325.00

Virus isolation/high stringency (8 weeks)

$400.00