Viral / Molecular Core Virology Services
Core Services Main Page | Virology | Molecular | Sequencing | SGA Services | BSL-3 Facility | Other
- HIV-1 viral isolate repository
- Virus isolation from clinical samples
- Virus phenotyping (SI / NSI and R5 / R5X4 / X4 analysis)
- HIV-1 p24 and SIV/HIV-2 p27 Gag antigen ELISA assay
- Amplification and QA of virus stocks
- Mycoplasma testing of virus stocks
- Inactivated HIV-1 Virions
- Plate Fluorimetry, Luminometry and Continuous Absorbance Spectophotometer
- Custom Viral Services & Consultation in other viral services
Core Service Contact:
Farida Shaheen, Ph.D.
Technical Director, Viral and Molecular Core
Tel: 215-898-5180
Fax: 215-573-4446
Email: farida@mail.med.upenn.edu
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Virus isolation from clinical samples
The Core will isolate HIV-1 from plasma, PBMC, fluids, or tissues provided by CFAR investigators (under approval of his/her human subjects IRB approval, if appropriate). Isolation can be done using either standard PBMC coculture methods or high stringency CD8-depleted cells. Cultures are typicaly maintained for up to 6 weeks.
Isolates will be provided as a tissue culture supernatant seed
stock. Stocks are quantified by p24 antigen content and confirmed
mycoplasma-free before distribution.
Arrangements should be made in advance for isolation set-up using the Isolation Request Form and calling 215-898-5180.
Fees: |
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Item |
Non-CFAR |
CFAR |
Virus Isolation - PBMC or plasma |
$190.00 |
$150.00 |
Virus isolation - tissue or other source |
$250.00 |
$200.00 |
Virus isolation/high stringency |
$315.00 |
$250.00 |