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Penn Center for AIDS Research

Viral / Molecular Core Virology Services

Core Services Main Page | Virology | Molecular | Sequencing | SGA Services | Intergrated vDNA | Other

 

 

Core Service Contact:
Farida Shaheen, Ph.D.
Technical Director, Viral and Molecular Core
Tel: 215-898-5180
Fax: 215-573-4446
Email: farida@mail.med.upenn.edu


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Inactivated HIV-1 Virions

Virions are grown in permissive cell lines or generated by transfection of 293T cells with proviral clones, and then treated with aldrithiol-2 (AT-2), which covalently modifies the zinc fingers of Gag to render virus non-infectious but preserves conformational and functional integrity of virion surface proteins. Virion particles are suitable for use where conformationally intact inactivated virus is desired.

While virus is tested for infectivity prior to release, we recommend that appropriate biosafety procedures be maintained nonetheless.

AT2-Treated Viruses

NL4-3/AT-2

89.6/AT-2
YU2/AT-2
JRCSF/AT-2
Bald (Env-negative) virus
Reference for AT-2 treatment: Rossio JL, et al. JV 72:7992-8001, 1998

Other custom HIV-1 or SIV viruses can be generated upon request; please inquire for details.