- HIV-1 viral isolate repository
- HIV-1 p24 and SIV/HIV-2 p27 Gag antigen ELISA assay
- HIV-1 viral load assay for plasma (research assay)- NEW!
- Inactivated HIV-1 Virions
- Amplification and QA of virus stocks
- Mycoplasma testing of virus stocks
- Virus isolation from clinical samples
- Virus phenotyping (SI / NSI and R5 / R5X4 / X4 analysis)
- Plate Fluorimetry, Luminometry and Continuous Absorbance Spectophotometer
- Custom Viral Services & Consultation in other viral services
HIV Viral Load assay for research purposes:
This assay is available for research purposes and is particularly suitable for use with small volumes of plasma obtained from humanized mouse studies. This is a real-time PCR assay that targets highly conserve region of HIV-1 Pol for quantification of viral RNA, and demonstrates superior performance compared to other gene targets when used with a broad range of group M viruses from different clades (including B, C and others).
For mouse plasma, the VL results will be provided as HIV-1 RNA copies per 100ul plasma. The optimal amount of plasma for the assay is 35 ul, which offers a limit of detection (LOD) of 10 copies per 100ul; it can be run on lower volumes with LOD adjustment as appropriate. Samples are run in duplicate, with internal controls including exogenous spike with unrelated virion RNA and no-RT controls to confirm complete virion recovery and the absence of inhibitors. Assay is run in batched format (10 samples per batch).
Customers should contact core to discuss in advance about the experimental details, such as viral strain used for infection, volume of plasma sample required, total sample etc.
- Plasma should be collected using EDTA as an anticoagulant; if collected using anticoagulant other than EDTA then please contact core to discuss this in advance (we have a protocol suitable for use on heparin plasma but must be informed in advance).
- While this assay is suitable for any plasma source, most investigators prefer that samples from human subjects be run on standard CLIA-approved platforms so that results can feed back to clinicians if deemed appropriate.
Order Virology Support services through the Path BioResource at Penn. Instructions for the Path BioResource can be found here on the CFAR website or on the Path BioResource site itself.
Core Service Contact:
Farida Shaheen, Ph.D.
Technical Director, Viral and Molecular Core
Top: Infection of CD4+ cell line with NSI isolate (left) and SI isolate (right), under phase microscopy.
Bottom: Immunofluorescence staining of HIV antigens (yellow). Uninfected cells (left) and infected cells (right).