Transgenic and Chimeric Mouse Facility

Core Description: The Transgenic and Chimeric Mouse Facility  provides a centralized service to efficiently produce infection-free transgenic, chimeric, and genome-edited strains of mice. These mice carry randomly inserted transgenes and/or site-specific alterations in the mouse genome of specific interest to Penn researchers. The Facility offers services including DNA pronuclear injection into fertilized oocytes (along with genotyping of transgenic founders), ES cell injection into blastocysts, cytoplasmic/pronuclear injections into fertilized oocytes of CRISPR-Cas9 mix (gRNA, Cas9RNA, ssDNA/dsDNA templates), embryo and sperm cryopreservation, in vitro fertilization, and re-derivation of live and cryopreserved lines.  The Core also oversees a cyropreservation facility for long-term storage of mouse embryos and sperm samples.  

Contact

Core Directors:
Stephen A. Liebhaber, M.D. 
Director 
215-898-7834
liebhabe@pennmedicine.upenn.edu 

Jean Richa, Ph.D. 
Technical Director
215-898-6064 (office) or 215-573-3023
jricha@pennmedicine.upenn.edu