Biomedical Research Core Facilities
Perelman School of Medicine at the University of Pennsylvania

Transgenic & Chimeric Mouse Facility 

Core Description: The Transgenic & Chimeric Mouse Facility provides a centralized service to efficiently produce infection-free transgenic founder and chimeric strains of mice carrying transgenes or gene "knockouts" of specific interest to Penn researchers.  The Facility offers services including DNA pronuclear injection into fertilized oocytes (along with genotyping of transgenic founders), ES cell injection into blastocysts, laser-assisted introduction of ES cells into 8-cell embryos, embryo and sperm cryopreservation, in vitro fertilization, and re-derivations of live and cryopreserved lines.  The Core also oversees a cyropreservation facility for long-term storage of mouse embryos and sperm samples.


Core Directors:
Nancy Cooke, M.D.

Stephen A. Liebhaber, M.D.

Technical Director:

Jean Richa, Ph.D.
215-898-6064 (office) or 215-573-3023