Research
Interests:
Dr. Storey's research interests have focused
primarily on the biochemistry of mammalian sperm function
and the mechanism by which sperm establish binding to the
egg zona pellucida. His work has provided a foundation for
many subsequent investigations by others dealing with the
analysis of specific forms of male infertility, the
development of techniques for the evaluation of sperm
function and the development of new approaches to
contraception. His early work on sperm mitochondrial
function and the localization of the enzymes of glycolysis
has had a significant impact on present studies relating to
the regulation of sperm function and the development of the
assisted reproductive technologies. His laboratory was the
first to demonstrate that the physiological acrosome
reaction was induced by the zona pellucida subsequent to
the binding of the sperm to this unique extracellular
matrix. This work has led to a basic understanding of the
fertilization process in mammals. His work on sperm lipid
peroxidation has identified the mechanisms by which sperm
viability is compromised and has led to the development of
new media to extend such viability. Finally, his most
recent work on sperm cryopreservation has provided the
basis for the development of new media for cryopreservation
of sperm from rare genetic strains of mice.
Dr. Storey has served the scientific community in numerous
ways. He has served on the editorial board of several
journals, has been a member of the Reproductive Biology
Study Section, served as the Chair of the Gordon Research
Conference on Fertilization and Activation of Development,
and has been a member of multiple professional and
scientific societies, including the Executive Council of
the American Society of Andrology. Perhaps on of the most
significant impacts that Bayard has had on the scientific
community has been his total commitment to the mentoring of
students, post-docs and junior faculty. The contributions
by Bayard to the scientific community have been
acknowledged by young investigators, his scientific peers
and by the American Society of Andrology with his election
as the 2000 Distinguished Andrologist.
Dr. Storey's active laboratory research was discontinued in
1999 pursuant to a move from 3rd floor John Morgan Bldg to
13th floor BRB II/III. Writeup of research results from the
laboratory continued until early 2001. Areas of research
covered in those studies may be inferred from the list of
publications below. Present activity includes acting as
reviewer for J Andrology, Biology of Reproduction, Human
Reproducton Update, Cryobiology, Reproduction, Molecular
Reproduction & Development, Molecular Human
Reproduction; collaboration with colleagues on papers and
grant applications.
Selected Publications:
Storey, B.T.: Biochemistry of the induction and prevention
of lipoperoxidative damage in human spermatozoa. Mol. Human
Reprod. 3: 203-213, 1997.
Noiles, E.E., Thompson, K.A., and Storey, B.T.: Water
permeability, Lp, of the mouse sperm plasma membrane and
its activation energy are strongly dependent on interaction
of the plasma membrane within the sperm cytoskeleton.
Cryobiology 35: 79-92, 1997.
Storey, B.T., Alvarez, J.G., and Thompson, K.A.: Human
sperm glutathione reductase activity in situ reveals
limitation in the glutathione antioxidant system due to
supply of NADPH. Mol. Reprod. Dev. 49: 400-407, 1998
Storey, B.T., Noiles, E.E., and Thompson, K.A.: Comparison
of glycerol, other polyols, trehalose, and raffinose to
provide a defined cryoprotectant medium for mouse sperm
cryopreservation. Cryobiology 37: 46-68, 1998.
Rockwell, P.L., and Storey, B.T.: Determination of the
intracellular dissociation constant, KD, of the fluo-3 Ca2+
complex in mouse sperm for use in estimating intracellular
Ca2+ concentrations. Mol. Reprod. Dev. 54: 418-428, 1999.
Rockwell, P.L., Storey, B.T.: Kinetics of onset of mouse
sperm acrosome reaction induced by solubilized zona
pellucida: fluorimetric determination of loss of pH
gradient between acrosomal lumen and medium monitored by
Dapoxyl (2-aminoethyl) sulfonamide and of intracellular
Ca2+ changes monitored by fluo-3. Mol. Reprod. Dev. 55:
335-349, 2000.
Travis, A.J., Jorgez, C.J., Merdiushev, T., Jones, B.H.,
Dess, D.M., Diaz-Cueto, L., Storey, B.T., Kopf, G.S., Moss,
S.B.: Functional relationships between
capacitation-dependent cell signaling and compartmentalized
metabolite pathways in murine spermatozoa. J. Biol. Chem.:
276: 7630-7636, 2001.
Thompson, K.A., Richa, J., Liebhaber, S.A., Storey, B.T.:
Dialysis addition of trehalose/glycerol cryoprotectant
allows recovery of cryopreserved mouse sperm with
satisfactory fertilizing ability as assessed by yield of
live young. J. Androl. 22: 339-344, 2001.
Selak, M.A., Storey, B.T., Peterside, I., Simmons, R.A.:
Impaired oxidative phosphorylation in skeletal muscle of
intrauterine growth-retarded rats. Am. J. Physiol.
Endocrinol. Metab. 285: E130-E137, 2003.
Galantino-Homer, H.L., Florman, H.M., Storey, B.T.,
Dobrinski, I., Kopf, G.S.: Bovine sperm capacitation:
assessment of phosphodiesterase activity and intracellular
alkalinization on capacitation-associated protein tyrosine
phosphorylation. Mol. Reprod. Dev. 67: 487-500, 2004.
