The CVI has interdisciplinary basic, translational, and clinical research core labs available to all its faculty members.
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The Cardiac Myocyte Core is a component of the Myocyte Biology and Heart Failure program unit of the Penn CVI.
This core is designed as a central resource for assisting CVI investigators with experiments requiring isolated cardiac myocytes.
Services offered include provision of neonatal rodent myocytes for cell culture experiments, isolation of adult myocytes from a variety of mammalian species,
isolated myocyte morphometric analyses and in vitro physiological studies examining cell shortening and responses to agonists.
Upon request, interested investigators can receive training that will allow them to perform cell isolations or analyses independently.
The Core Director is Xiaoyin Shan, Ph.D., under the supervision of Dr. Ken Margulies, M.D.
Core Director: Xiaoyin Shan, Ph.D. | xiaoyins@mail.med.upenn.edu
The Cardiovascular Genetics Core Laboratory is directed by Dr. Daniel L. Dries, MD, MPH,
and provides services to members of the Cardiovascular Institute. We offer medium-throughput genotyping services utilizing the Sequenom Mass Array Compact System and iPLEX technology.
This platform allows multiplex genotyping at the 24-plex level and is ideally suited for candidate gene association studies and fine mapping.
We assist researchers in the in the design of multiplex assays from a set of candidate SNPs.
In general, high-plexed assays can be designed for > 95% of all confirmed SNPs.
The core does not provide genomic DNA preparation services, but rather works with genomic DNA samples provided by the researchers in 96-well plates.
The core lab is equipped with several multi-channel liquid handlers to facilitate sample preparation and genotyping throughput.
Core Director: Dr. Daniel L. Dries, M.D., MPH | daniel.dries@uphs.upenn.edu, (215) 573-2781
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The core web site contains documents, protocols and suggestions relevant to the procedures performed in the Histology and Gene Expression Core Facility.
The Core provides expert professional services for members of the Cardiovascular Division. Services for non-division investigators is available on a fee-for-service basis as time permits.
Core Director: Dr. Min Min Lu | mlu2@mail.med.upenn.edu
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The CDB/CVI Microscopy Core is a full-service facility serving the entire University of Pennsylvania community.
Our aim is to provide personalized assistance on all aspects of imaging, from tips on sample preparation to training on one of our microscopes to processing and analysis of image data.
Our facility currently houses five light microscopes, a scanning electron microscope, and several computers dedicated to image processing and analysis.
Core Director: Andrea Stout | astout@mail.med.upenn.edu
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The Mouse Cardiovascular Physiology and Microsurgery Core is a component of Molecular Cardiology Research Center under the direction of Dr. Tao Wang.
This core facility is dedicated to aid investigators with a central resource for creating mouse models of cardiovascular disease, which provides a unique and valuable research opportunity
for translational research from animal studies to humans. We offer a wide variety of invasive surgical procedures and noninvasive ultrasound imaging techniques for comprehensive evaluation
of cardiovascular phenotypes of transgenic and knockout mice that may be difficult or time-consuming for those investigators without proper expertise, experience and equipments.
In addition, we offer microsurgery trainings and technique assistance for faculty, staff, fellows and students whose work requires microsurgical manipulations.
Core services are available to all Penn Cardiovascular Institute investigators.
Core Director: Dr. Tao Wang, M.D., Ph.D. | wangtao@mail.med.upenn.edu, (215) 898-2656
The Physiology Core within the Penn Cardiovascular Institute (Penn CVI) allows appropriate Penn CVI
investigators to perform in vivo physiologic cardiovascular analysis of small animal models including rats and mice.
Equipment and protocols developed within the core allow investigators to measure real time heart rate and rhythm using implantable
telemetry devices. Surface electrocardiograms and invasive electrophysiological testing is performed under the direction of
Dr. Vic Patel. Invasive
hemodynamic monitoring of both left and right heart allows for direct measurements of cardiovascular pressures and function. Ischemia reperfusion models
have been developed and validated using coronary ligation and fluorescent microsphere techniques to assess "myocardium at risk."
In combination with the Histology and Gene Expression Core Facility histologic analysis
of infracted myocardium is performed. Carotid injury models to examine vascular restenosis have also been developed. Micro pump implantation
to deliver constant infusion of substances including isoproteranol and angiotension II are also performed. Equipment within the core includes intubation and anesthesia apparati, telemetry
recording instruments, electrophysiology recording devices and appropriate electrical and pressure recording catheters.
Core Director: Dr. Vic Patel, M.D., Ph.D. | patelv@mail.med.upenn.edu, (215) 898-5475
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The Molecular Cardiology Research Center Mouse Transgenic and Knockout Core Facility generates transgenic and knockout mice for members of the
Center by performing pronuclear and blastocyst injecetions of DNA and embryonic stem cells, respectively.
Investigators interested in submitting samples for injection should present research objectives at the weekly Core meeting held Mondays at 10AM.
The Core functions under the direction of Diane Zhou, with help from Feiyan Liu and Wendy Li, under the supervision of
Dr. Edward Morrisey.
Core Director: Diane Zhou | dianez@mail.med.upenn.edu, (215) 573-3913
