News Update: Roche/454 GS FLX sequencing service has been launched at the DNA Sequencing Facility, School of Medicine. Users are requested to contact Tapan Ganguly (gangulyt@mail.med.upenn.edu).
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NOTE: DNA Sequencing costs only $6.00 per sample for investigators at the School of Medicine, the Cancer Center, and the Genomics Institute. The turnaround is about 24 hrs. High throughput DNA Sequencing is done at 50% cost. We also offer bisulphite sequencing and LOH (loss of heterozygosity) analysis.
Our latest offer is extraction of DNA from mammalian cells including ES cells, and bacterial plasmids grown in 96-well plates.
- Automated Cycle Sequencing
- Sequencing Method, Request Form (PDF), New Sample Submission Guide (PDF)
- High Throughput Sequencing
- Description, Request Form (PDF)
- Denaturing HPLC
- Description, Request Form (PDF)
- PCR, PCR Clean up, Cloning, Sub-cloning, Mutagenesis
- Description, Request Form (PDF)
- Large Sequencing Projects
- Description, Request Form (PDF)
- Template Preparation & Purification
- Description, Request Form (PDF)
- DNA Extraction from Mammalian Cells
- Description, Request Form (PDF)
- Genotyping/Fragment Sizing
- Description, Request Form (PDF)
- Targeting Vector Construction
- Description, Request Form (PDF)
- Sequence Analysis & Database Searches
- Description
For technical comments & questions: dnaseq@mail.med.upenn.edu
For Genetics Web Project feedback: genetics@www.med.upenn.edu