Charges for Services
» Template Preparation/Processing» DHPLC Wave System
» Microsatellite-based Genotyping
» High Throughput Sequencing
DNA Sequencing Facility Charges for Services -- Effective 7/1/2009
| DNA Sequencing | SOM, Cancer Center, PGI Members |
Other Penn, CHOP, Wistar |
*Off Campus (non-profits) |
|---|---|---|---|
| Full Sequencing | $6 | $6.65 | $9 |
| Ready to Load, RTL (electrophoresis only) | $3.15 | $3.50 | n/a |
| Ready to Load, Full plate or Half plate only (dried) | $2.25 | $2.50 | n/a |
| Enhancer (additional reagent) | $0.60 |
*NEW Bisulphite Sequencing |
Contact Staff | ||||
High Throughput Sequencing |
See Below | ||||
Sequencing Project (Primer Walk or Transposon mediated) |
Contact Staff | ||||
Sequence Analysis/Assembly |
Training is available at no charge. |
Template Preparation/Processing
| DNA Sequencing | SOM, Cancer Center, PGI Members | Other Penn, CHOP, Wistar | Off Campus (non-profits) | |
|---|---|---|---|---|
*NewDNA extraction from mammalian cells in 96-well plates |
$70/plate | $77/plate | $85/plate |
|
| Plasmid Miniprep on Qiacube (Qiagen) | $4.50/sample | $5/sample | $5.50/sample |
|
| Plasmid or phage Midiprep (1-3 samples) | $36/sample | $40/sample | $44/sample |
|
| Plasmid or phage Midiprep (4 or more samples) | $25.20/sample | $28/sample | $31/sample |
|
| Plasmid or phage Maxiprep (1-3 samples) | $45/sample | $50/sample | $55/sample |
|
| Plasmid or phage Maxiprep (4 or more samples) | $36/sample | $40/sample | $44/sample |
|
| Plasmid or phage Mega prep (1-3 samples) | $81/sample | $90/sample | $99/sample | |
| Plasmid or phage Mega prep (4 or more samples) | $45/sample | $50/sample | $55/sample |
|
| Plasmid or phage Maxiprep (4 or more samples) | $64.80/sample | $72/sample | $80/sample |
|
| PCR amplification of template (1-3 samples) | $10.80/sample | $12/sample | $13.20/sample |
|
| PCR amplification of template (4 or more samples) | $6.80/sample | $7.50/sample | $8.25/sample |
|
| *PCR product clean up(Enzymatic)- Reduced | $1.80/sample | $2/sample | $2.20/sample |
|
| *PCR product purification (column) - Reduced | $10.80/sample | $12/sample | $13.20/sample |
|
| *PCR product purification (gel) - Reduced | $20/sample | $22/sample | $24.20/sample |
|
| Transformation | $27/sample | $30/sample | $33/sample |
|
| *PCR product purification (gel) - Reduced | $20/sample | $22/sample | $24.20/sample | |
| Transformation | $27 | $30 | $33 |
|
| Site-directed Mutation (Mutagenic Oligos not included) | $225 | $250 | $275 |
|
| TA Cloning of PCR product (PCR amplification, DNA mini prep not included) | $36 | $40 | $44 |
|
| Sub-cloning (PCR, DNA mini prep, sequence verification not included) | $131 | $145 | $160 | |
| Targeting Vector Construction for Gene Targeting | Contact Staff | |||
Microsatellite-based Genotyping/Fragment Sizing on ABI 3100*(Price slashed)
| DNA Sequencing | SOM, Cancer Center, PGI Members | Other Penn, CHOP, Wistar | Off Campus (non-profits) |
|---|---|---|---|
| 96 samples (labeled PCR products submitted), w/genotyping | $100 | $110 | $121 |
| 48 samples (labeled PCR products submitted), w/genotyping | $64 | $70 | $77 |
| 96 samples RTL (samples+loading mix on ABI plate), w/genotyping | $64 | $70 | $77 |
| 48 samples RTL (samples+loading mix on ABI plate), w/genotyping | $40 | $44 | $49 |
| Full Service Genotyping (Includes PCR, pooling, sizing and genotyping) | Contact Staff | ||
| *New LOH Analysis | Contact Staff | ||
| Mouse Genotyping (for transgene identification) | Contact Staff | ||
High Throughput Sequencing
| DNA Sequencing | SOM, CC & PGI Members | Other PENN, CHOP, Wistar Investigators | Off Campus (non-profits) | |
|---|---|---|---|---|
| Regular Price | $6/sample | $6.65/sample | $9/sample | |
High Throughput Sequencing of user submitted PCR products or plasmid DNA
(Prescribed amount of DNA and primer are to be put into each well of a 96-well PCR plate, compatible with the ABI PCR machine. Sample sheet typed on an excel template is to be e-mailed as an attachment.)
| DNA Sequencing | SOM, Cancer Center, PGI Members | Other Penn, CHOP, Wistar | Off Campus (non-profits) | |
|---|---|---|---|---|
| Full Plate (96 samples) 1-5 plates/month |
$300 | $333 | n/a | |
| Full
Plate(96 samples), 6-10 plates/month |
$270 | $300 | n/a | |
| Full
Plate(96 samples), >11 plates/month |
$243 | $270 | n/a | |
High Throughput Sequencing from Bacterial Cultures (for high copy plasmids only)
(We will prepare plasmid DNA from cultures submitted in a plate and sequence. We can also prepare a culture plate from a glycerol stock plate. Sample sheet typed on an excel template is to be e-mailed as an attachment.)
| DNA Sequencing | SOM, Cancer Center, PGI Members | Other Penn, CHOP, Wistar | Off Campus (non-profits) | |
|---|---|---|---|---|
| Half Plate, one end sequenced | $260 | $285 | n/a | |
| Half Plate, both ends sequenced | $340 | $375 | n/a | |
| Full Plate, one end sequenced | $370 | $405 | n/a | |
| Full Plate, both ends sequenced) | $520 | $570 | n/a | |