DNA extraction from ES cells and mammalian cells grown in 96-well plates
Description of Service
DNA Sequencing Facility is pleased to announce DNA extraction service from ES cells and mammalian cells grown in 96-well plates. The extraction service would be of significant help to the investigators involved in gene targeting in mice. The service also would be valuable to other investigators involved in high throughput screening (HTS) using cultured cells.
The Agencourt Genfind method is used to extract DNA from cultured mammalian cells in 96-well plates. The method utilizes paramagnetic bead technology for rapid extraction of DNA without vacuum filtration or centrifugation. The method has been set up on a Beckman FX robot belonging to the Microarray facility. Starting from 0.5 – 2 x 105 cells/well, DNA is finally eluted into water. Two 96-well plates can be extracted at the same time. The yield is about 2 – 4 µg depending on the number of cells/well. Several laboratories at Penn have used this service so far and have successfully used the DNA for both PCR and southern blotting.
Cost : $200/plate. The cost is likely to go down with more usage.The facility is also in the process of setting up DNA extraction from plasmids grown in 96-well plates for high throughput screening using Agencourt’s paramagnetic bead technology.
For more information please contact Tapan Ganguly at 215-573-7238 or gangulyt@mail.med.upenn.edu