Cell Center Services

Hybridoma Production and Screening:

The Cell Center Service Facility will assist with monoclonal antibody production, including antigen preparation, immunization, fusion, screening and cloning. Our high efficiency fusion protocol typically results in about 80% hybridoma containing wells per fusion. Immunization is outsourced, however injections are performed to our specifications. Isolation of spleen cells, fusion and all following cell culture work is done at the Cell Center Service Facility.

Please contact Sabine Baxter for more information.

Immunization (outsourced)…………………………………………$400.00

2 mice – up to 5 injections per mouse – 1 spleen removal  - isolation of spleen cells

$150 for each additional mouse

Fusion (in-house)

Lymphocytes from 1 spleen into 10 x 96-well plates……………$180.00

Lymphocytes from 1 spleen into 20 x 96-well plates……………$250.00

Fusion maintenance and harvesting of supernatants for testing (in-house)

10 plates…………...………………………………………………………$500.00

20 plates………………………………………………………..…………$1000.00

Screening of supernatants by ELISA

10 plate fusion……………………………………………………………$1000.00

20 plate fusion……………………………………………………………….$2000.00

Expansion of positive clones – charge per clone………………….$20.00

Into 2 wells of a 24-well plate and freezing of 2 vials per clone

Subcloning by limiting dilution – charge per original clone…...$100.00

2 subclones per original clone – each subclone expanded into 2 wells of a 24-well plate – 2 vials frozen for each subclone

Request Form (word.doc)