Cell Center Services

Large scale mammalian or insect cell culture

The CCS will grow mammalian or insect cells for monoclonal antibody production, recombinant protein production or to harvest large numbers of cells. To this end there are several options available:

1. Roller bottle culture

The CCSF can grow suspension or adherent cells in roller bottles and produce up to 10L of antibody/recombinant protein containing supernatant. Or we can provide the researcher with the cell pellet

2. CelLine Flask culture

Adherent or suspension cells can be grown in the CelLine flask. There are two different protocols that can be utilized. One is the 14/14 protocol, where cells are grown for two weeks in the flask and about 15ml of very concentrated supernatant will be harvested and given to the researcher after the two-week period. The other is the 7/21 protocol, where the researcher is provided with about 15ml of concentrated supernatant every week for as long as the researcher desires.

3. Hollow-fiber bioreactor

This method of growing cells is best when multi-milligram amounts of antibody or recombinant protein need to be produced. The hollow-fiber cartridge is inoculated with cells and cells are grown on a continuous basis. 20 - 70ml of highly concentrated supernatant are harvested every day (except on weekends) and frozen immediately. Proteins produced in the hollow-fiber bioreactor are typically more homogeneous and generally of higher quality than proteins produced in other systems. A hollow-fiber cartridge can be kept going for as long as the researcher desires.

4. Purification of antibody or tagged recombinant proteins

IgG is purified by Protein G chromatography on FPLC.

Please contact Sabine Baxter (215-898-2795) for details.