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The Penn Gene Targeting Service, in association with the DNA Sequencing Facility and the Transgenic and Chimeric Mouse Facility, provides a full DNA-to-mouse service that is available to all Penn researchers and all academic researchers outside. This includes design and construction of targeting vectors (vector construction is done at the DNA Sequencing facility), electroporation and selection of vectors into several different mouse ES cell lines and, together with the customer, isolation of ES cell DNA followed by Southern/PCR screening to identify correctly recombined clones. Recombinant clones are tested by the service for cytogenetic integrity and expanded for injection into blastocysts. Timing of the expansion is coordinated with the Transgenic and Chimeric Mouse Facility which performs the injection and produces the chimera. Prices are low compared to comparable commercial facilities. For more information please contact Dr. Tobias Raabe (raabe@mail.med.upenn.edu or 215-898-5762).
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