University of Pennsylvania
DNA Sequencing Facility
Sequencing Services
Sanger Sequencing on ABI 3730
- Sequencing Few Samples
- High Throughput Sequencing from 96-well Plates
- Sequencing Projects (Primer Walk)
- Sequencing Template Preparation guidelines
Pyrosequencing on Roche 454FLX
Ion Semiconductor Sequencing on Ion Torrent
Other Services
Genotyping and Fragment Analysis Services
- Genotyping and Fragment Analysis on ABI 3130XL
- DNA, RNA and Protein Analysis on Agilent 2100 Bioanalyzer
- Mouse Genotyping for Transgene Identification
Molecular Biological Services
- Template (Plasmid) Preparation Including Transformation and Purification
- PCR and PCR Clean Up Followed by Sequencing and/or Cloning
- AMPure Bead Purification of Amplicons and DNA Fragments
- Site-Directed Mutagenesis
- Cloning and Sub-Cloning
- Targeting Vector Construction
Bioinformatics
The DNA Sequencing Facility is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.
