Penn Genomics Analysis Core

AMPure bead purification of amplicons and DNA fragments

Agencourt (Beckman) AMPure XP bead-based DNA purification of amplicons efficiently removes unincorporated dNTPs, primers, primer dimers, salts and other contaminants, resulting in high quality DNA suitable for sensitive downstream applications such as sequencing, genotyping, PCR, and sub-cloning. Recovery: 70 - 90% of double stranded and single stranded DNA with fragment sizes 150 bp - 10 kbp. This is the recommended purification method during sample preparation in most protocols for various new generation sequencing platforms.

PCR, PCR/DNA Clean-up and Cloning Request Form

Penn Genomics Analysis Core is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.

abramson-hup

Announcements:
Collection boxes have now been put in the lobby of BRB next to the UPS box and the 1st floor lobby of TRC (near the FEDEX box at the back).  Samples for DNA (Sanger) sequencing can be dropped off into the boxes.  Submission instructions and more information here.


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