University of Pennsylvania
DNA Sequencing Facility

Agilent 2100 Bioanalyzer

DNA, RNA and protein analysis on Agilent 2100 bioanalyzer

The Agilent 2100 Bioanalyzer, a microfluidics-based platform is suitable for sizing, quantification and quality control of DNA, RNA, proteins and cells. A fast automated run on the system for 30-40 minutes delivers high quality digital data.

This miniaturized analytical instrument has many advantages over conventional techniques including improved data precision and reproducibility, short analysis times, and minimal sample consumption.

DNA Size and Quantity

DNA 1000 Chip

High resolution separation and quantification of DNA; highly sensitive DNA sample QC for next generation sequencing experiments.

The DNA kits are ideal for automated sizing and quantification of PCR fragments including multiplex PCR products, restriction digests or fragmented DNA with minimal sample consumption. All next-gen sequencing protocols require DNA libraries to be run on bioanalyzer for quality assessment and quantification.

RNA quality check with RIN

RNA Nano Chip

RNA analysis offering total RNA, mRNA and Small RNA including RIN algorithm (RNA Integrity Number)

The Agilent RNA kits with the patented RNA Integrity Number (RIN) enables rapid RNA quality assessment; ideal for fast, easy and precise integrity checks and sample quantitation before any RNA dependent application. All next-gen sequencing protocols require RNA libraries to be run on bioanalyzer for quality assessment and quantification.

Sample Submission
The DNA Sequencing Facility currently offers the DNA 1000, High Sensitivity DNA and RNA 6000 Pico assays with rapid turnaround time. If you are interested in an assay we do not offer, please contact the facility directly. Please see the table below to determine which assay is appropriate for your samples. Submitted samples should be diluted to a concentration within the range specified for the assay selected.

Assay

Size Range

Concentration (PCR)

Concentration (Lib)

# Samples

DNA 1000

25-1000 bp

0.1-50 ng/ul

<= 50 ng/ul

12

DNA High Sensitivity

50-7000 bp

5-500 pg/ul

0.1-10 ng/ul

11

RNA 6000 Pico

NA

0.2-5 ng/ul (total RNA)

0.5-5 ng/ul (mRNA)

11

Agilent 2100 Run Request Form

The DNA Sequencing Facility is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.

abramson-hup

Announcements:
Collection boxes have now been put in the lobby of BRB next to the UPS box and the 1st floor lobby of TRC (near the FEDEX box at the back).  Samples for DNA (Sanger) sequencing can be dropped off into the boxes.  Submission instructions and more information here.


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