University of Pennsylvania
DNA Sequencing Facility
DNA, RNA and protein analysis on Agilent 2100 bioanalyzer
The Agilent 2100 Bioanalyzer, a microfluidics-based platform is suitable for sizing, quantification and quality control of DNA, RNA, proteins and cells. A fast automated run on the system for 30-40 minutes delivers high quality digital data. This miniaturized analytical instrument has many advantages over conventional techniques including improved data precision and reproducibility, short analysis times, and minimal sample consumption.
DNA Size and quantity
High resolution separation and quantification of DNA; highly sensitive DNA sample QC for next generation sequencing experiments. The DNA kits are ideal for automated sizing and quantification of PCR fragments including multiplex PCR products, restriction digests or fragmented DNA with minimal sample consumption. All next-gen sequencing protocols require DNA libraries to be run on a bioanalyzer for quality assessment and quantification.
RNA quality check with RIN
RNA analysis offering total RNA, mRNA and Small RNA including RIN algorithm (RNA Integrity Number). The Agilent RNA kits with the patented RNA Integrity Number (RIN) enables rapid RNA quality assessment; ideal for fast, easy and precise integrity checks and sample quantitation before any RNA dependent application. All next-gen sequencing protocols require RNA libraries to be run on a bioanalyzer for quality assessment and quantification.
SDS-PAGE replacement for protein analysis
The fast and reliable way to determine the quantity and purity of proteins from Coomassie down to silver stain sensitivity. The Agilent Protein kits provide a fast and flexible way for the assessment of protein concentration, identity, and purity in a wide variety of samples. The lab-on-a-chip approach eliminates handling of SDS-PAGE slab gels, staining or imaging steps.
The DNA Sequencing Facility is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.