Charges for Services

» Template Preparation/Processing
» Microsatellite-based Genotyping
» High Throughput Sequencing

DNA Sequencing Facility Charges for Services -- Effective 03/01/12
SOM, CancerCenter, PGFI members Other Penn,CHOP, Wistar *Off Campus (non-profits)
DNA Sequencing
Full Sequencing $6.00 $6.65 *$9
Ready to Load, RTL (electrophoresis only)
 Full plate only $88/plate $98/plate $132/plate
Enhancer (additional reagent ) $0.60 $0.60 $0.60
*Price reduced
 Bisulphite Sequencing Contact Staff
High Throughput Sequencing See Below
Sequencing Project (Primer Walk/Transposon mediated) Contact Staff
Sequence Analysis/Assembly Training is available at no charge
Template Preparation/Processing
DNA extraction from  plasmids  in 96-well plates $175/plate $190/plate $205/plate
Plasmid Miniprep on Qiacube (Qiagen) $4.50/sample $5/sample $5.50/sample
Plasmid or phage Midiprep (1-3 samples) $39.60/sample $44/sample $48.40/sample
Plasmid or phage Midiprep (4 or more samples) $27.70/sample $30.80/sample $33.90/sample
Plasmid or phage Maxiprep (1-3 samples) $49.50/sample $55/sample $60.50/sample
Plasmid or phage Maxiprep (4 or more samples) $39.60/sample $44/sample $48.40/sample
Plasmid or phage Mega prep (1-3 samples) $89.10/sample $99/sample $108.90/sample
Plasmid or phage Mega prep (4 or more samples) $72/sample $80/sample $88/sample
PCR amplification of template (1-3 samples) $10.80/sample $12/sample $13.20/sample
PCR amplification of template (4 or more samples) $6.80/sample $7.50/sample $8.25/sample
PCR product clean up (Enzymatic) $1.80/sample $2.00/sample $2.20/sample
PCR product purification (column) $11.90/sample $13.20/sample $14.50/sample
PCR product purification (gel) $21.80/sample $24.20/sample $26.60/sample
Transformation $30 $33 $36.30
Site-directed Mutation, mutagenic Oligos & sequencing not included $239 $265 $292
TA Cloning of PCR product $40 $44 $48.50
(PCR amplification, DNA mini prep not included)
Sub-cloning $144 $160 $176
(PCR, DNA mini prep, sequence verification not included)
Targeting Vector Construction for Gene Targeting
Conditional Knock-out construct $3,800
Knock-in construct $3,200
Microsatellite-based Genotyping/Fragment Sizing on ABI 3100 
96 samples (labeled PCR products), w/genotyping      $115 $127 $140
48 samples (labeled PCR products), w/genotyping $73 $81 $89
96 samples RTL(samples+loading mix on ABI plate), w/genotyping $73 $81 $89
48 samples RTL(samples+loading mix on ABI plate), w/genotyping $46 $51 $56
Full Service Genotyping Contact Staff
(Includes PCR, pooling, sizing and genotyping)
LOH Analysis Contact Staff
Mouse Genotyping  (for transgene identification) $2.30/mouse tail $2.55/mouse tail
High Throughput  Sequencing
SOM, CC, PGI Members Other PENN, CHOP, Wistar Off Campus (non-profits)
Regular Price $6/sample $6.65/sample $9/sample
High Throughput Sequencing of user submitted PCR products or plasmid DNA
( Prescribed amount of DNA and primer are to be put into each well of a 96-well PCR plate, compatible with the ABI PCR machine. Requests are to be submitted on line.)
Full Plate(96 samples),               1-5 plates/month $330 $366 $403
Full Plate(96 samples),             6-10 plates/month $297 $330 n/a
Full Plate(96 samples),           >10 plates/month $268 $297 n/a
High Throughput Sequencing from Bacterial Cultures (for high copy plasmids only)
(We prepare plasmid DNA from cultures submitted in a plate and sequence. We can also prepare a culture plate from a glycerol stock plate. Requests are to be submitted on line.)
Full Plate, one end sequenced $504 $560 $616
Full Plate, both ends sequenced $666 $740 $814