The DNA Sequencing Facility

The DNA Sequencing Facility is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.

Introducing Ion Torrent Sequencing at Penn

Ion Torrent sequencing service is now being offered at the DNA Sequencing Facility using 314, 316 and 318 chip. Users are requested to contact Tapan Ganguly. Read More...

Attention DNA Seq. Fac. Users

From July 6, 2010 (Tuesday) we will no longer accept hand written request forms for sequencing. Please pick up an instruction sheet if you are not using the web-based method for submission of sequencing samples. If you forget to submit request on line you will be able to use one computer in the facility for another 2 weeks. We strongly encourage everyone to use his/her lab computer to avoid delay.

Using any computer you can go to the facility web site http://www.med.upenn.edu/dnaseq/ and access the link http://www.med.upenn.edu/apps/my/dnacore/, log in with your PennKey and password, and follow the instructions (attached). The user can also go to this site directly.

Users from CHOP, Wistar and Monell and off-campus institutes please follow instructions.

For questions, comments please contact lab members.

Automated Cycle Sequencing »
Sequencing Method, Request Form (PDF), New Sample Submission Guide (PDF)

High Throughput Sequencing »
Description , Request Form (PDF)

PCR, PCR Clean up, Cloning, Sub-cloning, Mutagenesis »
Description, Request Form (PDF)

Large Sequencing Projects »
Description, Request Form (PDF)

Template Preparation & Purification »
Description, Request Form (PDF)

DNA Extraction from Mammalian Cells »
Description, Request Form (PDF)

Genotyping/Fragment Sizing »
Description, Request Form (PDF)

Targeting Vector Construction »
Description, Request Form (PDF)

Sequence Analysis & Database Searches »
Description

Roche 454Sequencing
Description Request Form (word.doc)

Ion Torrent Sequencing »
Description Request Form (word.doc)