University of Pennsylvania
DNA Sequencing Facility
Mouse Genotyping for Transgene Identification
Rapid genotyping of transgenic mice is highly important for successful breeding as well as for reducing the cost of strain maintenance. Particularly for large and multiple litters one needs to have the genotyping performed quickly in a cost-effective manner.
We offer a fast and convenient service for large scale mouse genotyping using Sigma REDExtract-N-Amp Tissue PCR kit starting from tail biopsies or ear punches. The user provides the mouse tails, control DNA with the transgene in it, and the PCR primers for the transgene together with any special PCR protocol. Two PCRs are done for each sample, one for the transgene and the other a control mGAPDH fragment. The amplicons are run on agarose gel and the labeled gel electrophoresis picture is provided within 48 hrs.
The DNA Sequencing Facility is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.