University of Pennsylvania
DNA Sequencing Facility
PCR, PCR Clean-Up & Cloning
PCR of genomic DNA or BAC DNA are done using custom primers. Primers can be designed at the facility. The optimization and the clean up of PCR products are done using various procedures including exo-sap, column and gel purification. The cleaned up products can be directly processed for Sanger sequencing at the facility or used for cloning.
PCR products are cloned using Invitrogen's TA cloning system.
Also see our AMPure XP purification service.
The DNA Sequencing Facility is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.