Penn Genomics Analysis Core

Pricing

Sanger Sequencing

Scale SOM, Cancer Center, PGFI members Other Penn, CHOP, Wistar Off Campus (non-profit)
Per Sample $6.50 $7.20 $9.00
Full Plate (< 6 / mo)1 $365 $405 $450
Full Plate (≥ 6 / mo)1 $330 $365 n/a
Full Plate (> 10 / mo)1 $300 $330 n/a
Ready to Load (full plate) $100 $112 $150
Sequencing Enhancer $.70 $.70 $.70
Bisulphite Sequencing Contact Facility
Sequencing Project Contact Facility
Sequence Analysis/Assembly Contact Facility
Sequencing from Bacterial Cultures
Full Plate, One End Sequenced2
$540 $600 $660
Sequencing from Bacterial Cultures
Full Plate, Both Ends Sequenced2
$715 $795 $875

1 Prescribed amount of DNA and primer are to be put into each well of a 96-well PCR plate, compatible with the ABI PCR machine. Requests are to be submitted on line.
2 For high copy plasmids only. We prepare plasmid DNA from cultures submitted in a plate and sequence. We can also prepare a culture plate from a glycerol stock plate. Requests are to be submitted on line.


454 Sequencing

Scale gDNA / Amplicon > 1.5kb Amplicons ≤ 1 kb
Full plate (1 sample) $10,000 $9,200
1/2 plate $5,400 $4,900
1/4 plate $3,180 $2,680
Barcoded Library Contact Facility


Ion Torrent Sequencing

Sequencing Chip 314 316 318
*  gDNA (& also long amplicons, includes 1 library prep) $600 (200b)
$725 (400b)
$800 (200b) $995 (200b)
§  Amplicons (library with adaptors already made) $515 (200b)
$640 (400b)
$720 (200b) 920 (200b)
* AmpliSeq Cancer panel v2 (50 cancer genes, 2,800 COSMIC mutations, includes 1 library prep) $700 (200b) $900 (200b)
Contact us for multiplexing
AmpliSeq Comprehensive Cancer & other panels Contact Facility
Barcoded library - $80 (gDNA, long amplicons, 4 minimum) $170 (for AmpliSeq cancer or custom panel, 4 minimum)


Genotyping and Fragment Analysis on ABI 3130XL

Scale SOM, Cancer Center, PGFI members Other Penn, CHOP, Wistar Off Campus (non-profit)
96 samples1 $133 $146 $161
48 samples1 $85 $93 $103
96 samples (RTL)1, 2 $85 $93 $103
48 samples (RTL)1, 2 $54 $59 $66
Full Service Genotyping Contact Facility
LOH Analysis Contact Facility

1 Customer provides labeled PCR products. Price includes software based genotyping.
2 Ready to Load


DNA, RNA and Protein Analysis on Agilent 2100 Bioanalyzer

SOM, Cancer Center, PGFI members Other Penn, CHOP, Wistar Off Campus (non-profit)
Bioanalyzer DNA1000, ≤ 12 samples $45 Contact Facility
Bioanalyzer DNA High Sensitivity, ≤ 11 samples $65 Contact Facility
Bioanalyzer RNA Pico, ≤ 12 samples $75 Contact Facility


Mouse Genotyping for Transgene Identification

SOM, Cancer Center, PGFI members Other Penn, CHOP, Wistar Off Campus (non-profit)
$2.60/tail $2.90/tail Contact Facility


Molecular Biology Services

SOM, Cancer Center, PGFI members Other Penn, CHOP, Wistar Off Campus (non-profit)
DNA extraction from plasmids in 96 well plate $175 $190 $205
Plasmid Miniprep on Qiacube $4.50 $5 $5.50
Plasmid or phage Midiprep (1-3 samples) $46 $51 $56
Plasmid or phage Midiprep (> 4 samples) $32 $35.50 $39
Plasmid or phage Maxiprep (1-3 samples) $57 $63.20 $70
Plasmid or phage Maxiprep (> 4 samples) $45.60 $51 $56
Plasmid or phage Megaprep (1-3 samples) $103 $114 $125
Plasmid or phage Megaprep (> 4 samples) $83 $92 $101
PCR amplification of template (1-3 samples) $10.80 $12 $13.20
PCR amplification of template (> 4 samples) $7.80 $8.60 $9.50
PCR product / fragment purification (Ampure) $7.50 $8.25 $9.10
PCR product purification (column) $8 $8.80 $9.70
PCR product purification (enzymatic) $2 $2.20 $2.40
PCR product purification (gel) $25 $28 $30.60
Transformation $35 $38 $41.75
Site-directed Mutation1 $263 $292 $320
TA Cloning of PCR product2 $60 $66 $72.60
Sub-cloning3 $166 $184 $205

1 Mutagenic oligos & sequencing not included
2 PCR amplification, DNA mini prep not included
3 DNA mini prep andsequence verification not included


Targeting Vector Construction

SOM, Cancer Center, PGFI members Other Penn, CHOP, Wistar Off Campus (non-profit)
Conditional knock-out construct $4,350
Knock-in construct $3,650


Bioinformatics1

SOM, Cancer Center, PGFI members Other Penn, CHOP, Wistar Off Campus (non-profit)
$100 / hour

1 For Roche 454 and Ion Torrent PGM data only

Penn Genomics Analysis Core is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.

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Announcements:
Collection boxes have now been put in the lobby of BRB next to the UPS box and the 1st floor lobby of TRC (near the FEDEX box at the back).  Samples for DNA (Sanger) sequencing can be dropped off into the boxes.  Submission instructions and more information here.


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