University of Pennsylvania
DNA Sequencing Facility
Sequence Analysis/Database Searches
Sequence Assembly & Analysis
Assembly of sequences into a contig, identification of ORFs, analysis of properties of the predicted protein, and other analyses can be performed at the facility using MacVector, Sequencher and other software.
These services are included at no charge for large sequencing projects. Training on the use of MacVector and Sequencher is provided free to the investigators.
The network licenses of MacVector, Sequencher and other desktop software are available from the Bioinformatics group of the Penn Molecular Profiling Facility.
BLAST, BLOCKS, BLITZ, PROSITE and FASTA searches are performed using WWW or e-mail servers at no charge. Please contact the facility to arrange for database searches or for training. Genomic sequences can also be processed to identify interspersed repeats, low complexity regions, GRAIL exons, transcription factor binding sites, frequency of mono, di, and tri nucleotides, and other features. Genomic sequences can also be submitted to the GAIA genome annotation system at the Bioinformatics Laboratory. Facility staff will also submit sequences to Genebank if requested by the investigator.
The DNA Sequencing Facility is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.