Penn Genomics Analysis Core

Site-Directed Mutagenesis

Mutagenesis including point mutation, insertion and deletion of single or multiple amino acids and large insertions are done using Stratagene's QuickChange Lightning kit. This includes design and synthesis of the mutagenic primers, mutagenesis and verification by sequencing. After mutageneis the DNA can be maxi prepped if requested. Simultaneous mutagenesis at different sites (Multisite mutations) can also be done using Stratagene's multi site-directed kit.

Site Directed Mutagenesis Request Form

Penn Genomics Analysis Core is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.

abramson-hup

Announcements:
Collection boxes have now been put in the lobby of BRB next to the UPS box and the 1st floor lobby of TRC (near the FEDEX box at the back).  Samples for DNA (Sanger) sequencing can be dropped off into the boxes.  Submission instructions and more information here.


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