University of Pennsylvania
DNA Sequencing Facility
Mutagenesis including point mutation, insertion and deletion of single or multiple amino acids and large insertions are done using Stratagene's QuickChange Lightning kit. This includes design and synthesis of the mutagenic primers, mutagenesis and verification by sequencing. After mutageneis the DNA can be maxi prepped if requested. Simultaneous mutagenesis at different sites (Multisite mutations) can also be done using Stratagene's multi site-directed kit.
The DNA Sequencing Facility is an Abramson Cancer Center Shared Resource that is approved and partially funded by the National Cancer Institute.