Charges for Services

The full cost of the standard transgenic services (DNA/RNA/ES) is $5,270.00 per construct/cell line. A discounted fee of $1,550.00 is available to Medical School investigators, who are members of the Abramson Cancer Center, Diabetes & Endocrinology Research Center, and Center for Molecular Study of Digestive and Liver Diseases, reflecting a substantial subsidy by the P30 grants supporting those organizations. For all other Penn and CHOP investigators, the cost of either service is $3,100.00 per construct/cell line.

DNA Microinjection

Currently, we harvest fertilized eggs from superovulated B6SJLF1/J females mated with B6SJLF1/J males (from JAX). We will attempt to accommodate requests for other mouse strains if required by specific experiments. The use of additional strains will incur added costs, reflecting the cost of the mice to be used.

ES Cell Microinjection

Currently, we harvest blastocysts from C57BL/6 females mated with C57BL/6 males (from Charles River). An initial, one time fee of $530.00 is charged for testing the non-recombinant ES cell line for its ability to contribute to chimerism.

RNA Microinjection

For all members of the 3 supporting centers, we will charge a fee of $1,550 per RNA mix injected for the production of genome-edited mice.  Currently, we harvest eggs from superovulated B6SJLF1/J females mated with B6SJLF1/J males (from JAX).  We will attempt to accommodate requests for other mouse strains if required by specific experiments.  However, additional animal costs will be incurred, reflecting the cost of the strain to be used.

Embryo Cryopreservation

For embryo cryopreservation, the cost will depend primarily on the successful reproduction of the breeding males provided and the set-up that you choose to follow. A minimum estimate of $775.00 to $1,550.00 is projected, for all members of the 3 supporting centers, with the cost of the quarantine housing being the responsibility of the investigator.

Mouse Line Rederivation

For line rederivation, the cost will depend primarily on the reproductive capabilities of the breeders available, and on the source location as well as the destination of the mouse line. A minimum estimate of $775.00 is projected for all members of the 3 supporting centers.

Sperm Cryopreservation and In-Vitro Fertilization

For Sperm Cryopreservation and In-Vitro Fertilization, the cost will depend primarily on the availability and location of the male mice. A minimum estimate of $775.00 per service is projected for all members of the 3 supporting cenmters.