Charges for Services
The full cost of the transgenic service (DNA/ES) is $5,270.00 per construct/cell line. The low fee of $1,550.00 for Medical School investigators, and members of the Cancer Center, Diabetes Center, and Digestive and Liver Diseases Center, is possible due to a substantial subsidy by those organizations. For all other Penn investigators, the cost of either service is $3,100.00 per construct/cell line.
We will charge a fee of $1,550.00 per DNA construct injected for the production of transgenic mice. The initiation of zygote injections depends upon receipt of an approved protocol and request form accompanied with properly labeled tubes of the DNA construct and documentation of its purity. Currently, we harvest fertilized eggs from superovulated B6SJLF1/J females mated with B6SJLF1/J males (from JAX). We will attempt to accommodate requests for other mouse strains if required by specific experiments. Please justify these requirements in your protocol for review. Additional costs will be incurred. In the case of DNA injection for transient transgenics, the fee will be $1,240.00 per DNA construct.
ES Cell Microinjection
For ES cell injection, a fee of $1,550.00 will be charged per cell line injected for the production of chimeric mice. The initiation of blastocysts injections depends upon receipt of an approved protocol and request form, following a schedule prearranged with Dr. Jean Richa. The schedule will be discussed after testing the ES cell line and approving the documentation of the recombination event in the assigned ES recombinant clone. Currently, we harvest blastocysts from C57BL/6J females mated with C57BL/6J males (from Charles River). An initial, one time fee of $530.00 is charged for testing the non-recombinant ES cell line for its ability to contribute to chimerism.
For embryo cryopreservation, the cost will depend primarily on the successful reproduction of the breeding males provided and the set-up that you choose to follow. A minimum estimate of $775.00 to $1,550.00 is projected, with the cost of the quarantine housing being the responsibility of the investigator.
Mouse Line Rederivation
For line rederivation, the cost will depend primarily on the reproductive capabilities of the breeders available, and on the source location as well as the destination of the mouse line. A minimum estimate of $775.00 is projected.
Sperm Cryopreservation and In-Vitro Fertilization
For Sperm Cryopreservation and In-Vitro Fertilization, the cost will depend primarily on the availability and location of the male mice. A minimum estimate of $775.00 per service is projected.