At the present time, the TCMF provides the following services on a routine basis:
DNA Injection »
Injection of DNA into mouse zygotes (fertilized oocytes) for the production of transgenic mice or embryos.
ES cell Injection »
Injection of embryonic stem cells into blastocysts (3.5-day embryo) for the production of chimeric mice.
RNA Injection »
Injection of a mix of Cas9 RNA/prot., gRNA and/or ssDNA/dsDNA into mouse zygotes for the production of site-directed genome-edited mice.
Mouse Line Rederivation »
Surgical transfer of embryos into pathogen-free recipient females, where they can develop to term.
Embryo Cryopreservation »
Controlled freezing of mouse embryos for preservation in liquid nitrogen.
Sperm Cryopreservation »
An alternate and less expensive procedure to preserve important mouse lines.
In-Vitro Fertilization »
Through this process, a mouse line that undergoes reproductive obstacles can be rescued.