Gene Therapy Program

Expression Vectors- by gene

GENIE: Calcium Sensor: GCaMP6

Deisseroth: Red-Shifted Optical Excitation: C1V1(E122T/E162T)

Deisseroth: Stabilized Step Function Opsins [SSFO]: ChR2 (C128S/D156A)

Cre-Dependent Expression Vectors- by gene

Allen Institute: Cre-Dependent Control Fluorophore

Looger: Cre-dependent Glutamate Sensor: iGluSnFr

Deisseroth: Cre-dependent Red-Shifted Optical Excitation C1V1(E122T/E162T)

Cre- Vectors - by Gene or Gene Fusion

Cre

Expression Vectors- by promoter

TBG (liver-specific)

cTNT (heart-specific)

Expression Vectors- by promoter-gene

CMV- eGFP

CAG(CB7)- mCerulean

CAG(CB7)- TurboRFP

CAG(CB7)- tdTomato

EF1a- eGFP

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1- GENIE acknowledgement (GCaMP5G): The Genetically-Encoded Neuronal Indicator and Effector (GENIE) Project and the Janelia Farm Research Campus of the Howard Hughes Medical Institute has generously allowed this material to be distributed with the understanding that requesting investigators need to acknowledge the GENIE Program and the Janelia Farm Research Campus  in any publication in which the material was used, specifically Loren L. Looger, Ph.D., Jasper Akerboom, Ph.D., Douglas S. Kim, Ph.D. from the GENIE Project, Janelia Farm Research Campus, Howard Hughes Medical Institute.

2- L. Looger/HHMI acknowledgement: Loren L. Looger, Ph.D. and Janelia Farm Research Campus of the Howard Hughes Medical Institute has kindly provided this material for distribution to the research community and should be acknowledged in any publication resulting from the use of the material

3- MTA for original source material: An MTA for the original source material is required. An MTA can be obtained through Addgene or directly from the original providing laboratory / institution. Confirmation of an MTA must be provided to the Vector Core prior to release of the material.

4- K. Deisseroth / Stanford MTA: An MTA for the original source material is required. An MTA or Permission can be obtained directly from the Deisseroth laboratory at Stanford. (Note: for Deisseroth laboratory materials provided through Addgene, see # 3). Confirmation of an MTA must be provided to the Vector Core prior to release of the material.

5- P. Haydon / Tufts acknowledgement: Philip G. Haydon and Douglas A. Coulter and the University of Pennsylvania have kindly provided this material for distribution to the research community and should be acknowledged in any publication resulting from the use of the material.

6- E. Boyden / MIT acknowledgement: Ed Boyden, Ph.D. and the Massachusetts Institute of Technology has kindly provided this material for distribution to the research community and should be acknowledged in any publication resulting from the use of the material

7- Allen Institute MTA Amendment: An MTA Amendment for the original source material is required (follow the link to locate the appropriate amendment form)

8- R. Campbell / U.Alberta acknowledgement: Robert Campbell, Ph.D. and the University of Alberta, Canada, has kindly provided this material for distribution to the research community and should be acknowledged in any publication resulting from the use of the material. Dr. Campbell may be contacted directly for the use of vectors based on additional materials generated in his laboratory.

9- L. Lagnado/Cambridge acknowledgement: Leon Lagnado, Ph.D. and Cambridge University has kindly provided this material for distribution to the research community and should be acknowledged in any publication resulting from the use of the material.

10- GENIE acknowledgement (GCaMP6): The Genetically-Encoded Neuronal Indicator and Effector (GENIE) Project and the Janelia Farm Research Campus of the Howard Hughes Medical Institute have generously allowed these materials to be distributed with the understanding that requesting investigators need to acknowledge the GENIE Program and the Janelia Farm Research Campus  in any publication in which the material was used, specifically Vivek Jayaraman, Ph.D., Rex A. Kerr, Ph.D., Douglas S. Kim, Ph.D., Loren L. Looger, Ph.D., Karel Svoboda, Ph.D. from the GENIE Project, Janelia Farm Research Campus, Howard Hughes Medical Institute.

11- J. Sanes / Harvard acknowledgement: Joshua R. Sanes Ph.D. and Dawen Cai Ph.D. and the Center for Brain Science at Harvard University have kindly provided this material for distribution to the research community and should be acknowledged in any publication resulting from the use of the material.

AAV AD HELPER PLASMID

AAV REP CAP PLASMIDS (TRANS PLASMIDS)