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Penn Vector Core: Services and Rates

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The Penn Vector Core has over a decade of experience in viral based vector production and currently offers vectors based on AAV, adenovirus and lentiviruses.

All of the vectors produced in our facility are:

Our core is the only vector core to establish release criteria that must be met by the vectors produced in our facility. If we do not meet the release criteria we have established for our vectors, we will repeat the preparation once again at not additional charge to the investigator. If the production of a vector must be repeated, the investigator will be billed only for the initial production and be provided with the preparation that resulted in the highest yield.

AAV Vector Services: A number of novel AAV vectors were originally isolated and developed at Penn in the laboratory of Dr. James Wilson and first made available to investigators through the Penn Vector Core. Due to its close proximity to the Wilson laboratory, the Penn Vector Core is able to rapidly assimilate new vector technologies and make them available to its users. The Core offers a variety of novel serotype AAV vectors and additional vectors currently under development will be distributed through the Penn Vector.

The Core currently offers 8 AAV vector serotypes, with additional serotypes also available. The tropism of selected AAV serotypes is shown in the following table with an X shown where efficacy has been demonstrated. For targets not listed, it may be necessary to conduct pilot serotype selection studies to determine the serotype of choice. We recommend serotype selection studies be performed in vivo since the in vitro transduction efficiencies of many of the serotypes below does not accurately reflect in vivo performance. For investigators wishing to discuss the serotype of choice for their application, please contact the Director of the Penn Vector Core.

AAV Serotype

Applications

(X = Recommended for use)
Skeletal muscle Heart Lung CNS / Eye Liver Adipose
AAV1
X
X
X
X
-
-
AAV2
-
-
-
X
-
-
AAV5
-
-
X
X
-
-
AAV6
X
X
X
-
X
X
AAV7
X
-
-
X
-
-
AAV8
X
-
-
X
X
X
AAV9
X
X
X
X
X
X
AAVrh10
-
-
X
-
-
-
Other novel AAV
-
-
-
-
-
-
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AAV Vector Rates*: The Penn Vector Core is pleased to offer 4 scales of AAV vector production services.

*Prices updated 10/1/13
Scale Volume Yield Reporter Custom External Academic Cost
aliquot
0.1 ml
5E+11 GC
X
 
$250
small
0.5 - 1ml
5E+12 GC
X
X
$1922
routine
1.5 - 2 ml
1E+13 GC
X
X
$2995
mega
4.5 - 6 ml
1E+14 GC
X
X
$9,404

 

Lentiviral Vector Rates*: The Penn Vector Core offers a single scale of lentiviral vector production

*Prices updated 10/1/13
Scale Volume Yield Reporter Custom External Academic Cost
aliquot
0.05 ml
1E+8 - E+9
X
 
$250
routine
0.5 - 1ml
1E+9 - E+10
X
X
$1908
routine
with RCL analysis
-
X
X
$2424

 

Adenoviral Vector Rates: Please contact the Vector Core for rates and quality control options

AAV Vector Release Criteria: The expected yields shown above are based on the average yields of reporter gene vectors generated for AAV serotypes 1, 5, 7, 8, 9 and rh10. (AAV serotypes 2 and 6 are somewhat lower yielding; please contact the vector core for expected yields.) Although we try our best to achieve the same yields for custom vector preparations as we do for our reporter gene vectors, our release criteria for custom vectors is ½ of the anticipated yield of our reporter gene preparations. If we do not meet the release criteria we have established for our vectors, we will repeat the preparation once again at not additional charge to the investigator. If the production of a vector must be repeated, the investigator will be billed only for the initial production and be provided with the preparation that resulted in the highest yield.

Our release criteria do not apply to AAV vectors with the following:

Vectors for which release criteria do not apply will be produced with no guarantee of yield.

AAV Vector Tools:

Rates for Support Service:

The rates for support services including cloning (one-step), DNA amplification (for routine scale production) and DNA structure and sequence analysis are provided in the table below. Note that DNA structure and sequence analysis services are required if in support of custom cloning services provided by the core and include full genome (eg. from AAV 5’ ITR to 3’ ITR) sequence analysis as well as thorough restriction enzyme analysis.

*Prices updated 10/1/13

Services Provided in Support of Custom Vector Production

External Academic
Rate

Clone New Plasmid (one-step)

$1,236

Plasmid DNA Production

$499

DNA Structure and Sequence Analysis

$632

 

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