Gene Therapy Program

Penn Vector Core: What's New?

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Update 10/25/12

Please click the following link for a complete list of what's new at the Penn Vector Core: What's New?

• AAV GCaMP6 Vectors
• AAV Glutamate Sensor Vectors
• AAV Optogenetic Vectors

Coming soon to the Penn Vector Core:

• AAV Serotype Selection Kits
• AAV ARGENT Vectors
• Online Ordering!

AAV GCaMP6 VECTORS

These vectors listed below can now be pre-ordered by completing an Inventory Request Form available on our Order Information page. AAV1 based vectors are currently in production and all are anticipated to be available for distribution by Nov 15. AAV5 based vectors are anticipated to be available by Dec 15 and AAV9 serotype vectors are anticipated to be available by Jan 15.

Please note: The Genetically-Encoded Neuronal Indicator and Effector (GENIE) Project and the Janelia Farm Research Campus of the Howard Hughes Medical Institute have generously allowed these materials to be distributed with the understanding that requesting investigators need to acknowledge the GENIE Program and the Janelia Farm Research Campus  in any publication in which the material was used, specifically Vivek Jayaraman, Ph.D., Rex A. Kerr, Ph.D., Douglas S. Kim, Ph.D., Loren L. Looger, Ph.D., Karel Svoboda, Ph.D. from the GENIE Project, Janelia Farm Research Campus, Howard Hughes Medical Institute.

If you have not ordered from our Core before, please visit our Order Information page for instructions and to download the following forms:

• Investigator Profile Form (2) Material Transfer Agreement and (2) Inventory Vector Request form and/or Custom Vector Request Form

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AAV Glutamate Sensor Vectors

The vectors listed in the table below can be pre-ordered by completing an Inventory Request Form available on our Order Information page. AAV1 based vectors are currently in production and are all anticipated to be available for distribution by Nov 15. AAV5 based vectors are anticipated to be available by Dec 15 and AAV9 serotype vectors are anticipated to be available by Jan 15.

Please note: Loren L. Looger, Ph.D. and the Janelia Farm Research Campus of the Howard Hughes Medical Institute have kindly provided these materials for distribution to the research community and should be acknowledged in any publication resulting from the use of the material.

If you have not ordered from our Core before, please visit our Ordering Information Page for instructions and to download the following forms:

• Investigator Profile Form (2) Material Transfer Agreement and (2) Inventory Vector Request form and/or Custom Vector Request Form

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AAV Optogenetic Vectors

The Core is working on making all of the optogenetic vectors listed on the What's New flyer available by Jan 15, 2013. The vectors currently available are designated with a ‘Y’ in the column under the AAV serotype indicated. We are also planning to make selected vectors available as a Serotype Selection Kit which will contain all the serotypes available for pilot studies to best determine the serotype of choice for a particular application.

Please note: Karl Deisseroth, M.D., Ph.D. and Stanford University have generously provided these materials for distribution to the research community and should be acknowledged in any publication resulting from the use of the material. In addition, laboratories receiving the materials listed below must hold an MTA with Stanford for the original source materials (plasmid DNA) used to generate the vectors. MTAs may be obtained directly from the Deisseroth lab at Stanford (please visit http://www.stanford.edu/group/dlab/optogenetics/request_dna.html for more information) or obtained through the Addgene website (www.addgene.org) for which there will be a $65 administrative fee per plasmid.

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