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The Genomics and Gene Targeting Core offers fee-for-service microarray experimental design, sample hybridization and analysis. The global aim of the Core is to provide our clients with a complete microarray package, from the initial steps of experimental design to the final steps of result interpretation and publication. The service we provide can be broken down into three main areas:
- Production of high quality microarrays for gene expression profiling. The Core manufactures and prints in house high density microarrays from human and mouse cDNAs or 70mer oligos, using stringent QA and QC measures. All array print runs are rigorously quality controlled by test hybridization to our standard probes. We also work with investigators to print custom arrays based upon the individual project requirements.
- Probe preparation and sample hybridization. The Core has developed highly sensitive methods for sample labeling, detection and microarray analysis. We can produce excellent data sets with as little as 5-25 ng total RNA, or the amount obtained from a single pancreatic islets. We offer a full range of hybridization services including assessment of sample quality, linear RNA amplification, sample labeling and hybridization, batch slide scanning, image quantification, data and pathway analysis. High throughput methods allow for the labeling and hybridization of up to 48 samples in a single day.
- Analysis of microarray data and training in expression profiling. The Core provides consultation and advice on microarray experimental design and analysis. Our aim is to work with all investigators prior to their providing samples, so that they can be trained in microarray experimental design, from the use of appropriate RNA isolation techniques to giving advice on data interpretation and publication of their experiment. We have developed significant technical expertise in the area of microarray analysis, and can provide data analysis and bioinformatics support as needed.
Through the support of the NIDDK Research Center grants we are able to offer both Institute for Diabetes, Obesity, and Metabolism (IDOM; P30DK19525) and Center for Molecular Studies in Digestive and Liver Diseases (CMSDLD; P30DK50306) members substantial discounts on the cost of these services. Please remember to acknowledge these centers and the services of the Genomics and Gene Targeting Core in any ensuing research publications.
For additional information or to set up an initial consultation, please contact:
Peter White
GGTC, Department of Genetics
570 Clinical Research Building
415 Curie Bouevard
Philadelphia , PA 19104-6145
Tel: +1 (215) 898-0773
Fax: +1 (215) 573-2326
E-mail: pwhite@mail.med.upenn.edu
