Functional Genomics Core - Genome Analyzer II
High Throughput Sequencing
The Illumina® Whole Genome Analyzer II offers us a unique opportunity to expand our service far beyond what can currently be achieved using traditional hybridization based approaches to analyze both expression and promoter occupancy studies. The traditional tools for this type of analysis are limited by the number of probes that can be spotted onto an array and by limited detection sensitivity and dynamic range. The Solexa creates a unique opportunity for investigators to generate datasets that will go far beyond these limits, as the system in simplest terms counts molecules of DNA, allowing investigators to detect single copy genes and entirely novel transcripts or regulatory regions for which traditional array probes do not exist. Furthermore, the Solexa® Sequencing by Synthesis technology employed by the system allows investigation of RNA or DNA from any species, and will allow investigation of species for which traditional arrays do not exist. This system can be utilized by researchers with interests in gene expression analysis, Whole-Genome Chromatin IP Sequencing (ChIP-Seq), DNA methylation studies, SNP analysis, micro RNA analysis, gene discovery and sequencing. The numerous application and power of this system will give Penn researchers a significant advantage in their field of study and enable them to publish in high impact journals.
Highlights of the technology
Using a massively parallel sequencing approach, the Illumina Genome Analyzer can generate more than one billion bases of data in a single run. The system leverages Solexa sequencing technology and novel reversible terminator chemistry, optimized to achieve unprecedented levels of cost effectiveness and throughput.
- Reduced project cost: Enables sequencing at 1% the cost of conventional technology
- Ultra-high throughput: Generates up to a billion bases of usable data per run—100x the throughput of conventional methods
- High accuracy: Delivers accurate, high-confidence data, even in homopolymeric regions
- Application versatility: From DNA sequencing to digital expression profiling and small RNA discovery, the Illumina Genome Analysis System enables you to take on a broad range of applications rapidly and economically.
- Coming Soon: GA IIx and Paired End Sequencing. The upgrade to GA IIx allows us to offer 20% more reads for the same cost. The Paired End Module will allow us to offer both 5' and 3' sequencing, for greater sequencing coverage of the sample. In addition, it allows us to offer barcoding for multiplex sequencing.
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