Functional Genomics Core - Next-Gen Sequencing
Illumina's High-Throughput Sequencers offers us a unique opportunity to expand our service far beyond what can currently be achieved using traditional hybridization based approaches to analyze both expression and promoter occupancy studies. The traditional tools for this type of analysis are limited by the number of probes that can be spotted onto an array and by limited detection sensitivity and dynamic range. The sequencers create a unique opportunity for investigators to generate datasets that will go far beyond these limits, as the system in simplest terms counts molecules of DNA, allowing investigators to detect single copy genes and entirely novel transcripts or regulatory regions for which traditional array probes do not exist. Furthermore, the Illumina® Sequencing by Synthesis technology employed by the system allows investigation of RNA or DNA from any species, and will allow investigation of species for which traditional arrays do not exist. This system can be utilized by researchers with interests in gene expression analysis, Whole-Genome Chromatin IP Sequencing (ChIP-Seq), DNA methylation studies, SNP analysis, micro RNA analysis, gene discovery and sequencing. The numerous application and power of this system will give DERC/IDOM and Penn researchers a significant advantage in their field of study and enable them to publish in high impact journals.
Genome Analyzer IIx
Using a massively parallel sequencing approach, the Illumina® Whole Genome Analyzer IIx can generate more than one billion bases of data in a single run or 45 million uniquely aligned reads per lane. The system leverages Illumina's sequencing technology and novel reversible terminator chemistry, optimized to achieve unprecedented levels of cost effectiveness and throughput.
- Reduced project cost: Enables sequencing at 1% the cost of conventional technology
- Ultra-high throughput: Generates up to a billion bases of usable data per run which is 100x the throughput of conventional methods. Can sequence up to 150bp.
- High accuracy: Delivers accurate, high-confidence data, even in homopolymeric regions.
- Application versatility: From DNA sequencing to digital expression profiling and small RNA discovery, the Illumina Genome Analysis System enables you to take on a broad range of applications rapidly and economically.
- GA IIx and Paired End Sequencing: The GA IIx allows us to offer more reads for the same cost. The Paired End Module will allow us to offer both 5' and 3' sequencing, for greater sequencing coverage of the sample. In addition, it allows us to offer barcoding for multiplex sequencing.
HiSeq 2000
The HiSeq 2000 sequencing system offers unprecedented output and a breakthrough user experience. HiSeq 2000 delivers the industry's highest sequencing output and fastest data generation rate. The HiSeq allows us to be more flexible in scheduling our 100bp PE runs and be able to accommodate our collaborators needs. Currently, the HiSeq generates around 300 billion bases per flowcell and over 200 million uniquely aligned reads per lane.
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