Pancreatic Islet Cell Biology Core
General Mission Statement:
The main goal of the Core is to serve as a resource for Center Investigators who are studying pancreatic islet cell biology by offering islet isolation and culture, functional phenotyping of islets and consultation to optimize use of core services. We also work with investigators to adapt existing technologies to solve unique problems that cannot be addressed by standard methodologies.
List of Services:
- Pancreatic islet isolation from mouse or rat.
- Islet perifusion to measure insulin, glucagon and cAMP release (in conjunction with the Radioimmunoassay/Biomarkers Core).
- Batch incubation experiments to measure insulin, glucagon and cAMP release (in conjunction with the Radioimmunoassay/Biomarkers Core).
- Quantitative oxygen consumption of perifused freshly isolated or cultured islet tissue using optical methods (requires 500-600 islets).
- Electrode based closed cell respirometry for measuring oxygen consumption of isolated islets or other tissues (e.g. fat cells) using a water-jacketed glass vessel (requires 150 islets).
- Respiration and glycolysis measured with the Seahorse XFe96 Extracellular Flux Analyzer.
- Free intracellular Ca2+ measurements using new instrumentation for state of the art fluorescence imaging (Zeiss Observer.Z1 with AxioVision softwere).
- Consultations on functional analysis of pancreatic islets and development of new strategies and technologies to solve particular problems.
In addition, the ICBC provides training for graduate students, postdoctoral fellows and research staff in the following fields:
- Pancreatic islet isolation and perfusion (Wei Qin);
- Basic principles of respiration and oxidative phosphorylation. Measurement of oxygen consumption by different methods including electrode based closed respirometry (Clark electrode), phosphorescence respirometry in perifusion system and oxygen consumption and glycolysis by Seahorse system (Dr. Doliba).
- Provide training in all aspects of islet cell physiology and biochemistry (Drs. Matschinsky, Stoffers, Doliba).
Please remember to acknowledge the Institute for Diabetes, Obesity and Metabolism, the DRC grant (P30DK19525) and the services of the Islet Cell Biology Core in any ensuing research publications.