Pancreatic Islet Cell Biology Core
General Mission Statement:
To serve as a resource for Center Investigators that are studying pancreatic islet cell biology by offering islet isolation and culture, functional phenotyping of islets and providing consultation and help to develop strategies how to use the services of the core optimally or attempt to modify available technologies to solve particular problems.
List of Services:
- Pancreatic islet isolation from mouse or rat.
- Islet perfusion to measure insulin, glucagon and cAMP release (in connection with the Radioimmunoassay Core). Batch incubation experiments to measure insulin, glucagon and cAMP release (in connection with the Radioimmunoassay Core).
- Respiration and glycolysis measured by Seahorse System.
- Performing closed cell respirometry based on measuring islet oxygen consumption in a water-jacketed glass vessel (requires 150 islets).
- Quantitating oxygen consumption of perifused freshly isolated or cultured islet tissue using optical methods (requires 500-600 islets).
- Investigating the behavior of free intracellular Ca2+ using state of the art fluorescence imaging techniques.
- Glucose usage and oxidation of cultured pancreatic islets
Please remember to acknowledge the Institute for Diabetes, Obesity and Metabolism, the DRC grant (P30DK19525) and the services of the Islet Cell Biology Core in any ensuing research publications.