Genomics and Gene Targeting Core

Genomics and Gene Targeting Core - Costs

Researchers note: Please remember to acknowledge the Institute for Diabetes, Obesity and Cardiovascular Metabolism, the DERC grant (P30DK19525) and the services of the Functional Genomics Core in any ensuing research publications.

Service Costs as of November 2004

Members of the Diabetes and Endocrinology Research Center or Center for Molecular Studies in Liver and Digestive Diseases receive a discount of up to 85% off of list price. Investigators outside of these centers receive a 50% discount off of list price. All commercial or for profit organizations will receive a 10% discount off list price for service orders containing 10 or more samples. To order arrays only, please go to: [www.betacell.org/microarrays]

Outline of Services and Associated Fees

Service Request Form (pdf 56 kb)

Name	Description	LIST PRICE	UPENN Academic	IDOM CMSDLD
Sample Preparation
RNA quality assessment	Up to 12 samples per run on Agilent Bioanalyzer	120	60	50
RNA amplification from 5-100 ng	Only required if less than 25 m g total RNA is available or RNA is precious.	200	100	80
Islet purity matching	All islet samples must be matched for purity using Q-PCR analysis.	70	35	30
RNA extraction - mini <100 m g RNA	Starting material: 0.5 - 30 mg or 10 to 1x10^7 cells	30	15	10
FGC Arrays NOTE: Agilent Arrays or other commercial platforms are priced according to manufacturers current pricing
PancChip 6 Mouse Array	13,000 mouse cDNAs known to be expressed in pancreas/islets	200	100	30
PancChip 1 Human Array	12,000 human cDNAs known to be expressed in pancreas/islets	200	100	30
Mouse PromoterChip BCBC-5A or 5B	Contains 18,000 amplicons of mouse promoter sequences.	200	100	50
Mouse MGH oligo array	14,000 mouse 70mer oligos representing ALL tissues	250	150	50
Expression Analysis NOTE: We have a minimum order fee of THREE biological replicates (SIX samples)
Sample labeling, hybridization	Includes labeling, hybridization, image and basic data analysis. Requires 10-50 m g total RNA (1 m g/ m l in water).	300	150	80
Die swap analysis	By averaging the ratio of expression with a die swap analysis, greater accuracy of the data will be achieved (Required if n <=4).	100	50	35
Common control	Time course or analysis of multiple treatment groups may require the use of a common control sample (optional).	100	50	35
Promoter Analysis NOTE: We have a minimum order fee of THREE biological replicates (SIX samples)
DNA Sample labeling & hybridization	Includes labeling, hybridization, image and data analysis. Requires 2 m g amplified DNA (0.1 m g/ m l in water)	300	150	80
Miscellaneous
Custom Array	Printing up to 100 slides of user provided DNA or oligos	6000	3000	2000
Array Development	Development of a custom array application	P.O.R	P.O.R	P.O.R