Immunology Graduate Group
Glen N. Gaulton, Ph.D.
Professor of Pathology/Lab Medicine
Vice Dean for Research and Research Training
Address: 345 Biomedical Research Building II/III,
421 Curie Blvd.
Philadelphia, PA 19104-6142
Office Phone: (215) 898-2875
Fax: (215) 573-7945
Email: gaulton@mail.med.upenn.edu
Education:
B.S., Biology, University of Southern California
M.S., Biochemistry and Molecular Biology, University of California, Santa Barbara
Ph.D., Biochemistry and Molecular Biology, University of California, Santa Barbara
Post Doctoral Fellow, Harvard Medical School
Research Interest
Lymphocyte development and retroviral pathology
Research Techniques:
Molecular genetics, cell biology, histology, basic immunology
Research Summary
The interests of the Gaulton laboratory focus on an increased understanding of the molecular processes that regulate the development and pathogenesis of T lymphocytes. The selection and clonal expansion of T lymphocytes is an essential component in both the development of protective immunity and the coordinate preservation of immunologic tolerance. The laboratory has defined processes fundamental to development of the human thymus. This includes definition of the functions of stromal and thymocyte elements in thymocyte selection and differentiation. We have developed exciting gene therapy techniques for analysis of normal thymic cell function, as well as to study the experimental manipulation of function to repair genetic defects and to combat autoimmunity. This work includes analysis of naturally occurring mutations, which result in X-linked severe combined immunodeficiencies.
The laboratory has also investigated the effects of human and murine retrovirus within the developing thymus and CNS. Recent results have identified the in vivo ablation of thymocyte development in HIV-1 infected neonates, and have established human thymic organ culture for ex vivo infection studies. The long-term goal of these studies is to repair HIV-1 defects within the thymus. We have also investigated the pathology of HIV and murine retroviruses for cell fusion and entry to immunologically privileged sites such as the CNS.
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| HIV-1 infection of human thymic macrophages. Human thymic macrophages were isolated from an HIV-1 infected subject, and detection of HIV-1 infection was achieved by staining with an anti-HIV-1 nef antibody. The presence of HIV-1 within these cells is indicated by a bright pattern on the cytoplasmic background. |
Key words: Lymphocyte Development, Retrovirus Pathology, HIV-1, Gene Therapy
Recent Publications
Rosenzweig, M., Bunting, E.M., Damico, R.L., Clark, D.P. and Gaulton, G.N. 1995. Human neonatal thymic organ culture: an ex vivo model of thymocyte ontogeny and HIV-1 infection. Pathobiology 62: 245-251.
Marshall, D.J., Park, B.H., Korostoff, J.M. and Gaulton, G.N. 1995. Manipulation of the immune response by foreign gene expression in the thymus. Leukemia. 9: 128-132.
Gaulton, G.N. Scobie, J. and Rosenzweig, M. 1997.¾ HIV-1 and the Thymus. AIDS. 11: 403-414.
Gaulton, G.N. 1998. Viral pathogenesis and immunity within the thymus. Immunology. 17: 75-82.
Majka, M., Rozmyslowicz, T., Lee, B., Honczarenko, M., Gaulton, G., Pietrzowski, Z., Poncz, M., Gewirtz, A.M., Silberstein, L. and Ratajczak, M.Z. 1999. R5 HIV Inhibitory b-Chemokines are Secreted by Human Bone Marrow CD34+ Cells and Interferon-g Upregulates their Expression. J. Clin. Invest. 104: 1739-1749.
Chung, M., Kizhatil, K., Albritton, L.M., and Gaulton, G.N. 1999. Induction of Syncytia by Neuropathogenic Murine Leukemia Viruses Depends on Receptor Density, Host Cell Determinants and the Intrinsic Fusion Potential of Envelope protein. J. Virology 73: 9377-9385.
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