Kaestner Lab

School of Medicine Department of Genetics

Protocols

Chromatin Immunoprecipitation and ChIP-Seq Suggestions

RNA Extraction from Mammalian Tissues

Indirect Immunofluorescence for Paraformaldehyde-Fixed Paraffin-Embedded Tissue

Indirect Immunofluorescence for Snap-frozen OCT-Embedded Tissues

Whole-mount immunostaining (immunofluorescence)

Screen ES cells by Southern Blot

Isolation of DNA from ES cells in 6-well plate

96 Well DNA Preparation

Electroporation of ES cells

Making Mouse Liver Chromatin

Summary of RNA in situ hybridization procedure using paraffin-embedded section

Islets isolation

Protocol for making in situ riboprobe

in situ hybridization using digoxigenin-labeled probes and paraffin-embedded slides

Filters, Plates

HANKS BUFFER with 5mM Glucose

Krebs-Henseleit Buffer Stocks

Header Image:
Deletion of Foxa2 in Foxa2 ^loxP/loxP; Foxa3Cre mice. Efficiency and timing of Foxa2 deletion by Foxa3Cre were evaluated by immunofluorescence. Expression of Foxa2 was assessed by immunofluorescence in the P3 pancreas. Insulin (green) and Foxa2 (red) were found in the control pancreas (left), while Foxa2 protein was completely deleted in both islets and acini in the Foxa2 ^loxP/loxP; Foxa3Cre pancreas (right).

University of Pennsylvania | School of Medicine