Kaestner Lab

School of Medicine Department of Genetics

Selected publications

• Bramswig NC, Kaestner KH.
  Epigenetics and diabetes treatment: an unrealized promise?
  Trends Endocrinol Metab. 2012 Mar 14.
  PMID: 22424897 [PubMed - as supplied by publisher]
  
  
• Li Z, Tuteja G, Schug J, Kaestner KH.
  Foxa1 and Foxa2 are essential for sexual dimorphism in liver cancer.
  Cell. 2012 Jan 20;148(1-2):72-83.
  PMID: 22265403 [PubMed - indexed for MEDLINE]
  
  
• Bramswig NC, Kaestner KH.
  Organogenesis and functional genomics of the endocrine pancreas.
  Cell Mol Life Sci. 2012 Jan 13.
  PMID: 22241333 [PubMed - as supplied by publisher]
  
  
• Friedman JR, Kaestner KH.
  On the origin of the liver.
  J Clin Invest. 2011 Dec;121(12):4630-3. doi: 10.1172/JCI59652. Epub 2011 Nov 21.
  PMID: 22105167 [PubMed - indexed for MEDLINE] PMCID: PMC3226340
  
  
• Shin S, Walton G, Aoki R, Brondell K, Schug J, Fox A, Smirnova O, Dorrell C, Erker L, Chu AS, Wells RG, Grompe M, Greenbaum LE, Kaestner KH.
  Foxl1-Cre-marked adult hepatic progenitors have clonogenic and bilineage differentiation potential.
  Genes Dev. 2011 Jun 1;25(11):1185-92. doi: 10.1101/gad.2027811.
  PMID: 21632825 [PubMed - indexed for MEDLINE] PMCID: PMC3110956
  
  
• Li Z, Schug J, Tuteja G, White P, Kaestner KH.
  The nucleosome map of the mammalian liver.
  Nat Struct Mol Biol. 2011 Jun;18(6):742-6. Epub 2011 May 29.
  PMID: 21623366 [PubMed - indexed for MEDLINE] PMCID: PMC3148658
  

• Soccio RE, Tuteja G, Everett LJ, Li Z, Lazar MA, Kaestner KH.
  Species-specific strategies underlying conserved functions of metabolic transcription factors.
  Mol Endocrinol. 2011 Apr;25(4):694-706. Epub 2011 Feb 3.
  PMID: 21292830 [PubMed - indexed for MEDLINE]
  
  
• McKenna LB, Schug J, Vourekas A, McKenna JB, Bramswig N, Friedman JR, Kaestner KH.
  MicroRNAs Control Intestinal Epithelial Differentiation, Architecture, and Barrier Function.
  Gastroenterology. 2010 Nov;139(5):1654-64, 1664.e1. Epub 2010 Jul 24.
  PMID: 20659473 [PubMed - as supplied by publisher]
  
  
• Kaestner KH.
  The FoxA factors in organogenesis and differentiation.
  Curr Opin Genet Dev. 2010 Oct;20(5):527-32. Epub 2010 Jun 28.
  PMID: 20591647 [PubMed - in process]
  
  
• Gao N, Kaestner KH.
  Cdx2 regulates endo-lysosomal function and epithelial cell polarity.
  Genes Dev. 2010 Jun 15;24(12):1295-305.PMID: 20551175
  [PubMed - indexed for MEDLINE]
  
  
• Gao N, Le Lay J, Qin W, Doliba N, Schug J, Fox AJ, Smirnova O, Matschinsky FM, Kaestner KH.
  Foxa1 and Foxa2 maintain the metabolic and secretory features of the mature beta-cell.
  Mol Endocrinol. 2010 Aug;24(8):1594-604. Epub 2010 Jun 9.
  PMID: 20534694 [PubMed - in process]
  
  
• Kaestner KH.
  From embryonic stem cells to beta-cells: new hope for diabetics?
  Gastroenterology. 2010 Jun;138(7):2211-2.
  PMID: 20435010 [PubMed - indexed for MEDLINE]
  
  
• Bhandare R, Schug J, Le Lay J, Fox A, Smirnova O, Liu C, Naji A, Kaestner KH.
  Genome-wide analysis of histone modifications in human pancreatic islets.
  Genome Res. 2010 Apr;20(4):428-33. Epub 2010 Feb 24.
  PMID: 20181961 [PubMed - indexed for MEDLINE]
  
  
• Kaestner KH.
  Converting intestine into esophagus: anterior-posterior patterning of the vertebrate gut requires Cdx2.
  Cell Cycle. 2010 Feb 15;9(4):634-5. Epub 2010 Feb 13.
  PMID: 20107317 [PubMed - indexed for MEDLINE]
  
  
• Kaestner KH.
  Making the liver what it is: the many targets of the transcriptional regulator HNF4alpha.
  Hepatology. 2010 Feb;51(2):376-7.
  PMID: 20101744 [PubMed - indexed for MEDLINE]
  
  
• Le Lay J, Kaestner KH.
  The Fox genes in the liver: from organogenesis to functional integration.
  Physiol Rev. 2010 Jan;90(1):1-22. Review.PMID: 20086072
  [PubMed - indexed for MEDLINE]
  
  
• May CL, Kaestner KH.
  Gut endocrine cell development.
  Mol Cell Endocrinol. 2010 Jul 8;323(1):70-5. Epub 2009 Dec 16. Review.
  PMID: 20025933 [PubMed - indexed for MEDLINE]
  
  
• Rieck S, Kaestner KH.
  Expansion of beta-cell mass in response to pregnancy.
  Trends Endocrinol Metab. 2010 Mar;21(3):151-8. Epub 2009 Dec 16. Review.
  PMID: 20015659 [PubMed - indexed for MEDLINE]
  
  
• Ye DZ, Kaestner KH.
  Foxa1 and Foxa2 control the differentiation of goblet and enteroendocrine L- and D-cells in mice.
  Gastroenterology. 2009 Dec;137(6):2052-62. Epub 2009 Sep 6.
  PMID: 19737569 [PubMed - indexed for MEDLINE]
  
  
• Le Lay J, Tuteja G, White P, Dhir R, Ahima R, Kaestner KH.
  CRTC2 (TORC2) contributes to the transcriptional response to fasting in the liver but is not required for the maintenance of glucose homeostasis.
  Cell Metab. 2009 Jul;10(1):55-62.PMID: 19583954
  [PubMed - indexed for MEDLINE]
  
  
• Rieck S, White P, Schug J, Fox AJ, Smirnova O, Gao N, Gupta RK, Wang ZV, Scherer PE, Keller MP, Attie AD, Kaestner KH.
  The transcriptional response of the islet to pregnancy in mice.
  Mol Endocrinol. 2009 Oct;23(10):1702-12. Epub 2009 Jul 2.
  PMID: 19574445 [PubMed - indexed for MEDLINE]
  
  
• Kaestner KH.
  In the zone: how a hepatocyte knows where it is.
  Gastroenterology. 2009 Aug;137(2):425-7. Epub 2009 Jun 30.
  PMID: 19573634 [PubMed - indexed for MEDLINE]
  
  
• Tuteja G, White P, Schug J, Kaestner KH.
  Extracting transcription factor targets from ChIP-Seq data.
  Nucleic Acids Res. 2009 Sep;37(17):e113. Epub 2009 Jun 24.
  PMID: 19553195 [PubMed - indexed for MEDLINE]
  

• White P, Kaestner KH.
  Gene expression analysis in diabetes research.
  Methods Mol Biol. 2009;560:239-61.
  PMID: 19504254 [PubMed - indexed for MEDLINE]
  
  
• Golson ML, Le Lay J, Gao N, Brämswig N, Loomes KM, Oakey R, May CL, White P, Kaestner KH.
  Jagged1 is a competitive inhibitor of Notch signaling in the embryonic pancreas.
  Mech Dev. 2009 Jun 6. [Epub ahead of print]
  PMID: 19501159 [PubMed - as supplied by publisher]
  
  
• Li Z, White P, Tuteja G, Rubins N, Sackett S, Kaestner KH.
  Foxa1 and Foxa2 regulate bile duct development in mice.
  J Clin Invest. 2009 Jun;119(6):1537-45. doi: 10.1172/JCI38201. Epub 2009 May 11.
  PMID: 19436110 [PubMed - in process]
  
  
• Bochkis IM, Schug J, Rubins NE, Chopra AR, O'Malley BW, Kaestner KH.
  Foxa2-Dependent Hepatic Gene Regulatory Networks Depend on Physiological State.
  Physiol Genomics. 2009 May 5. [Epub ahead of print]
  PMID: 19417011 [PubMed - as supplied by publisher]
  
  
• Gao N, White P, Kaestner KH.
  Establishment of intestinal identity and epithelial-mesenchymal signaling by Cdx2.
  Dev Cell. 2009 Apr;16(4):588-99.
  PMID: 19386267 [PubMed - indexed for MEDLINE]
  
  
• Hannenhalli S, Kaestner KH.
  The evolution of Fox genes and their role in development and disease.
  Nat Rev Genet. 2009 Apr;10(4):233-40. Review.
  PMID: 19274050 [PubMed - indexed for MEDLINE]
  
  
• Golson ML, Loomes KM, Oakey R, Kaestner KH.
  Ductal malformation and pancreatitis in mice caused by conditional Jag1 deletion.
  Gastroenterology. 2009 May;136(5):1761-71.e1. Epub 2009 Jan 23.
  PMID: 19208348 [PubMed - indexed for MEDLINE]
  
  
• Gao N, LeLay J, Vatamaniuk MZ, Rieck S, Friedman JR, Kaestner KH.
  Dynamic regulation of Pdx1 enhancers by Foxa1 and Foxa2 is essential for pancreas development.
  Genes Dev. 2008 Dec 15;22(24):3435-48.
  PMID: 19141476 [PubMed - indexed for MEDLINE]
  
  
• Madison BB, McKenna LB, Dolson D, Epstein DJ, Kaestner KH.
  FoxF1 and FoxL1 link hedgehog signaling and the control of epithelial proliferation in the developing stomach and intestine.
  J Biol Chem. 2009 Feb 27;284(9):5936-44. Epub 2008 Dec 2.
  PMID: 19049965 [PubMed - indexed for MEDLINE]
  
  
• Kaestner KH.
  A two-step pathway to resist fasting.
  Cell Metab. 2008 Dec;8(6):449-51.
  PMID: 19041759 [PubMed - indexed for MEDLINE]
  
  
• Bochkis IM, Rubins NE, White P, Furth EE, Friedman JR, Kaestner KH.
  Hepatocyte-specific ablation of Foxa2 alters bile acid homeostasis and results in endoplasmic reticulum stress.
  Nat Med. 2008 Aug;14(8):828-36. Epub 2008 Jul 27.PMID: 18660816 [PubMed - indexed for MEDLINE]]
  
  
• Tuteja G, Jensen ST, White P, Kaestner KH.
  Cis-regulatory modules in the mammalian liver: composition depends on strength of Foxa2 consensus site.
  Nucleic Acids Res. 2008 Jul;36(12):4149-57. Epub 2008 Jun 13.
  PMID: 18556755 [PubMed - in process]
  
  
• White P, May CL, Lamounier RN, Brestelli JE, Kaestner KH.
  Defining pancreatic endocrine precursors and their descendants.
  Diabetes. 2008 Mar;57(3):654-68. Epub 2007 Dec 10.
  PMID: 18071024 [PubMed - indexed for MEDLINE]
  
  
• Tuteja G, Kaestner KH.
  Forkhead transcription factors II.
  Cell. 2007 Oct 5;131(1):192. No abstract available.
  PMID: 17923097 [PubMed - indexed for MEDLINE]
  
  
• Gao N, White P, Doliba N, Golson ML, Matschinsky FM, Kaestner KH.
  Foxa2 controls vesicle docking and insulin secretion in mature Beta cells.
  Cell Metab. 2007 Oct;6(4):267-79.
  PMID: 17908556 [PubMed - indexed for MEDLINE]
  
  
• Tuteja G, Kaestner KH.
  SnapShot: forkhead transcription factors I.
  Cell. 2007 Sep 21;130(6):1160. No abstract available.
  PMID: 17889656 [PubMed - indexed for MEDLINE]
  
  
• Kaestner KH.
  Beta cell transplantation and immunosuppression: can't live with it, can't live without it.
  J Clin Invest. 2007 Sep;117(9):2380-2.
  PMID: 17786232 [PubMed - indexed for MEDLINE]
  
  
• Sackett SD, Fulmer JT, Friedman JR, Kaestner KH.
  Foxl1-Cre BAC transgenic mice: a new tool for gene ablation in the gastrointestinal mesenchyme.
  Genesis. 2007 Aug;45(8):518-22.
  PMID: 17661401 [PubMed - indexed for MEDLINE]
  

• Behr R, Sackett SD, Bochkis IM, Le PP, Kaestner KH.
  Impaired male fertility and atrophy of seminiferous tubules caused by haploinsufficiency for Foxa3.
  Dev Biol. 2007 Jun 15;306(2):636-45. Epub 2007 Apr 5. 
  PMID: 17488644 [PubMed - in process]
  

• Gupta RK, Gao N, Gorski RK, White P, Hardy OT, Rafiq K, Brestelli JE, Chen G, Stoeckert CJ, Kaestner KH.
  Expansion of adult beta-cell mass in response to increased metabolic demand is dependent on HNF-4alpha.
  Genes Dev. 2007 Apr 1;21(7):756-69.
  PMID: 17403778 [PubMed - indexed for MEDLINE]
  
  
• Hardy OT, Hohmeier HE, Becker TC, Manduchi E, Doliba NM, Gupta RK, White P, Stoeckert CJ, Matschinsky FM, Newgard CB, Kaestner KH.
  Functional genomics of the beta-cell: short-chain 3-hydroxyacyl-coenzyme A dehydrogenase regulates insulin secretion independent of K+ currents.
  Mol Endocrinol. 2007 Mar;21(3):765-73. Epub 2006 Dec 21.
  PMID: 17185391 [PubMed - indexed for MEDLINE]
  
  
• Vatamaniuk MZ, Gupta RK, Lantz KA, Doliba NM, Matschinsky FM, Kaestner KH.
  Foxa1-deficient mice exhibit impaired insulin secretion due to uncoupled oxidative phosphorylation.
  Diabetes. 2006 Oct;55(10):2730-6.
  PMID: 17003337 [PubMed - indexed for MEDLINE]
  
  
• Friedman JR, Kaestner KH.
  The Foxa family of transcription factors in development and metabolism.
  Cell Mol Life Sci. 2006 Oct;63(19-20):2317-28.
  PMID: 16909212 [PubMed - indexed for MEDLINE]
  
  
• Kaestner KH.
  Of cilia and cysts: modeling pancreatic polycystic disease.
  Gastroenterology. 2006 Mar;130(3):926-8.
  PMID: 16530530 [PubMed - indexed for MEDLINE]
  
  
• Kaestner KH.
  Collecting new targets in MODY.
  Cell Metab. 2005 Dec;2(6):342-4.
  PMID: 16330318 [PubMed - indexed for MEDLINE]
  
  
• Kaestner KH.
  The making of the liver: developmental competence in foregut endoderm and induction of the hepatogenic program.
  Cell Cycle. 2005 Sep;4(9):1146-8. Epub 2005 Sep 15.
  PMID: 16123587 [PubMed - indexed for MEDLINE]
  
  
• Phuc Le P, Friedman JR, Schug J, Brestelli JE, Parker JB, Bochkis IM, Kaestner KH
  .Glucocorticoid receptor-dependent gene regulatory networks.
  PLoS Genet. 2005 Aug;1(2):e16. Epub 2005 Aug 5.
  PMID: 16110340 [PubMed - indexed for MEDLINE]
  
  
• Zhang L, Rubins NE, Ahima RS, Greenbaum LE, Kaestner KH
  .Foxa2 integrates the transcriptional response of the hepatocyte to fasting.
  Cell Metab. 2005 Aug;2(2):141-8.
  PMID: 16098831 [PubMed - indexed for MEDLINE]
  
  
• Rubins NE, Friedman JR, Le PP, Zhang L, Brestelli J, Kaestner KH.
  Transcriptional networks in the liver: hepatocyte nuclear factor 6 function is largely independent of Foxa2.
  Mol Cell Biol. 2005 Aug;25(16):7069-77.
  PMID: 16055718 [PubMed - indexed for MEDLINE]
  
  
• Lee CS, Friedman JR, Fulmer JT, Kaestner KH. Related Articles, Links
  The initiation of liver development is dependent on Foxa transcription factors.
  Nature. 2005 Jun 16;435(7044):944-7.
  PMID: 15959514 [PubMed - in process]
  
  
• Katz JP, Perreault N, Goldstein BG, Actman L, McNally SR, Silberg DG, Furth EE, Kaestner KH.
  Loss of Klf4 in mice causes altered proliferation and differentiation and precancerous changes in the adult stomach.
  Gastroenterology. 2005 Apr;128(4):935-45.
  PMID: 15825076 [PubMed - indexed for MEDLINE]
  
  
• Gupta RK, Vatamaniuk MZ, Lee CS, Flaschen RC, Fulmer JT, Matschinsky FM, Duncan SA, Kaestner KH.
  The MODY1 gene HNF-4alpha regulates selected genes involved in insulin secretion.
  J Clin Invest. 2005 Apr;115(4):1006-15. Epub 2005 Mar 3.
  PMID: 15761495 [PubMed - in process]
  
  
• Wan H, Dingle S, Xu Y, Besnard V, Kaestner KH, Ang SL, Wert S, Stahlman MT, Whitsett JA.
  Compensatory roles of Foxa1 and Foxa2 during lung morphogenesis.
  J Biol Chem. 2005 Apr 8;280(14):13809-16. Epub 2005 Jan 24.
  PMID: 15668254 [PubMed - in process]
  
  
• Lantz KA, Kaestner KH.
  Winged-helix transcription factors and pancreatic development.
  Clin Sci (Lond). 2005 Mar;108(3):195-204. Review.
  PMID: 15631623 [PubMed - indexed for MEDLINE]
  
  
• Lee CS, Sund NJ, Behr R, Herrera PL, Kaestner KH.
  Foxa2 is required for the differentiation of pancreatic alpha-cells.
  Dev Biol. 2005 Feb 15;278(2):484-95.
  PMID: 15680365 [PubMed - indexed for MEDLINE]
  
  
• Perreault N, Sackett SD, Katz JP, Furth EE, Kaestner KH.
  Foxl1 is a mesenchymal Modifier of Min in carcinogenesis of stomach and colon.
  Genes Dev. 2005 Feb 1;19(3):311-5. Epub 2005 Jan 13.
  PMID: 15650110 [PubMed - indexed for MEDLINE]
  

• White P, Brestelli JE, Kaestner KH, Greenbaum LE.
  Identification of transcriptional networks during liver regeneration.
  J Biol Chem. 2005 Feb 4;280(5):3715-22. Epub 2004 Nov 16.
  PMID: 15546871 [PubMed - indexed for MEDLINE]
  

• Lee CS, Kaestner KH.
  Clinical endocrinology and metabolism. Development of gut endocrine cells.
  Best Pract Res Clin Endocrinol Metab. 2004 Dec;18(4):453-62. Review.
  PMID: 15533769 [PubMed - indexed for MEDLINE]
  

• Krupczak-Hollis K, Wang X, Kalinichenko VV, Gusarova GA, Wang IC, Dennewitz MB, Yoder HM, Kiyokawa H, Kaestner KH, Costa RH.
  The mouse Forkhead Box m1 transcription factor is essential for hepatoblast mitosis and development of intrahepatic bile ducts and vessels during liver morphogenesis.
  Dev Biol. 2004 Dec 1;276(1):74-88.
  PMID: 15531365 [PubMed - indexed for MEDLINE]

• Gupta RK, Kaestner KH.
  HNF-4alpha: from MODY to late-onset type 2 diabetes.
  Trends Mol Med. 2004 Nov;10(11):521-4. Review.
  PMID: 15519277 [PubMed - indexed for MEDLINE]

• Wan H, Xu Y, Ikegami M, Stahlman MT, Kaestner KH, Ang SL, Whitsett JA. Related Articles, Links
  Foxa2 is required for transition to air breathing at birth.
  Proc Natl Acad Sci U S A. 2004 Oct 5;101(40):14449-54. Epub 2004 Sep 27.
  PMID: 15452354 [PubMed - indexed for MEDLINE]

• Friedman JR, Larris B, Le PP, Peiris TH, Arsenlis A, Schug J, Tobias JW, Kaestner KH, Greenbaum LE. Related Articles, Links
  Orthogonal analysis of C/EBPbeta targets in vivo during liver proliferation.
  Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):12986-91. Epub 2004 Aug 18.
  PMID: 15317935 [PubMed - indexed for MEDLINE]

• Lantz KA, Vatamaniuk MZ, Brestelli JE, Friedman JR, Matschinsky FM, Kaestner KH. Related Articles, Links
  Foxa2 regulates multiple pathways of insulin secretion.
  J Clin Invest. 2004 Aug;114(4):512-20.
  PMID: 15314688 [PubMed - indexed for MEDLINE]

• Behr R, Brestelli J, Fulmer JT, Miyawaki N, Kleyman TR, Kaestner KH.
  Mild nephrogenic diabetes insipidus caused by Foxa1 deficiency.
  J Biol Chem. 2004 Oct 1;279(40):41936-41. Epub 2004 Jul 13.
  PMID: 15252040 [PubMed - indexed for MEDLINE]

• Katz JP, Perreault N, Goldstein BG, Chao HH, Ferraris RP, Kaestner KH.
  Foxl1 null mice have abnormal intestinal epithelia, postnatal growth retardation, and defective intestinal glucose uptake.
  Am J Physiol Gastrointest Liver Physiol. 2004 Oct;287(4):G856-64. Epub 2004 May 20.
  PMID: 15155178 [PubMed - indexed for MEDLINE]

• Wan H, Kaestner KH, Ang SL, Ikegami M, Finkelman FD, Stahlman MT, Fulkerson PC, Rothenberg ME, Whitsett JA.
  Foxa2 regulates alveolarization and goblet cell hyperplasia.
  Development. 2004 Feb;131(4):953-64.
  PMID: 14757645 [PubMed - indexed for MEDLINE]

• Kaestner KH, Lee CS, Scearce LM, Brestelli JE, Arsenlis A, Le PP, Lantz KA, Crabtree J, Pizarro A, Mazzarelli J, Pinney D, Fischer S, Manduchi E, Stoeckert CJ Jr, Gradwohl G, Clifton SW, Brown JR, Inoue H, Cras-Meneur C, Permutt MA.
  Transcriptional program of the endocrine pancreas in mice and humans.
  Diabetes. 2003 Jul;52(7):1604-10.
  PMID: 12829622 [PubMed - indexed for MEDLINE]

• Lee CS, Sund NJ, Vatamaniuk MZ, Matschinsky FM, Stoffers DA, Kaestner KH. Related Articles, Links
  Foxa2 controls Pdx1 gene expression in pancreatic beta-cells in vivo.
  Diabetes. 2002 Aug;51(8):2546-51.
  PMID: 12145169 [PubMed - indexed for MEDLINE]
  
  
• Lee CS, Perreault N, Brestelli JE, Kaestner KH.
  Neurogenin 3 is essential for the proper specification of gastric enteroendocrine cells and the maintenance of gastric epithelial cell identity.
  Genes Dev. 2002 Jun 15;16(12):1488-97.
  PMID: 12080087 [PubMed - indexed for MEDLINE]

• Katz JP, Perreault N, Goldstein BG, Lee CS, Labosky PA, Yang VW, Kaestner KH.
  The zinc-finger transcription factor Klf4 is required for terminal differentiation of goblet cells in the colon.
  Development. 2002 Jun;129(11):2619-28.
  PMID: 12015290 [PubMed - indexed for MEDLINE]

• Silberg DG, Sullivan J, Kang E, Swain GP, Moffett J, Sund NJ, Sackett SD, Kaestner KH.
  Cdx2 ectopic expression induces gastric intestinal metaplasia in transgenic mice.
  Gastroenterology. 2002 Mar;122(3):689-96.
  PMID: 11875002 [PubMed - indexed for MEDLINE]

• Perreault N, Katz JP, Sackett SD, Kaestner KH.
  Foxl1 controls the Wnt/beta-catenin pathway by modulating the expression of proteoglycans in the gut.
  J Biol Chem. 2001 Nov 16;276(46):43328-33. Epub 2001 Sep 12.
  PMID: 11555641 [PubMed - indexed for MEDLINE]

• Sund NJ, Vatamaniuk MZ, Casey M, Ang SL, Magnuson MA, Stoffers DA, Matschinsky FM, Kaestner KH.
  Tissue-specific deletion of Foxa2 in pancreatic beta cells results in hyperinsulinemic hypoglycemia.
  Genes Dev. 2001 Jul 1;15(13):1706-15.
  PMID: 11445544 [PubMed - indexed for MEDLINE]