Publications
Nabel CS, Jia H, Ye Y, Shen L, Goldschmidt HL, Stivers JT, Zhang Y, Kohli RM. AID/APOBEC deaminases disfavor modified cytosines implicated in DNA demethylation. Nat Chem Biol. 2012, doi: 10.1038/nchembio.1042. (PMID 22772155)
Nabel CS, Manning SA, Kohli RM. The curious chemical biology of cytosine: deamination, methylation, and oxidation as modulators of genomic potential. ACS Chem Biol. 2012 7:20-30. (PMID 22004246)
Nabel CS, Kohli RM. Demystifying DNA demethylation. Science. 2011, 333, 1229-30. (PMID 21885763)
Kohli RM. The Chemistry of a Dynamic Genome. Nat Chem Biol. 2010, 6, 866–868. (PMID: 21079591)
Kohli RM, Maul RW, Guminski AF, McClure RL, Gajula KS, Saribasak H, McMahon MA, Siliciano RF, Gearhart PJ, Stivers JT. Local sequence targeting in the AID/APOBEC family differentially impacts retroviral restriction and antibody diversification. J Biol Chem. 2010, 285:40956-64. (PMID: 20929867)
McMahon MA, Siliciano JD, Kohli RM, Siliciano RF. Sensitivity of V75I HIV-1 reverse transcriptase mutant selected in vitro by acyclovir to anti-HIV drugs. AIDS. 2010, 24:319-23. (PMID: 20009920)
Kohli RM, Abrams SR, Gajula KS, Maul RW, Gearhart PJ, Stivers JT. A portable hotspot recognition loop transfers sequence preferences from APOBEC family members to activation-induced cytidine deaminase. J Biol Chem. 2009, 284: 22898-22904. (PMID: 19561087)
Ho RS, Kohli RM, Maragakis LL. When to think of zebras. Am J Med. 2009, 122:424-6. (PMID 19375549)
McMahon MA, Siliciano JD, Lai L, Liu JO, Stivers JT, Siliciano RF, Kohli RM. The Anti-Herpetic Drug Acyclovir Inhibits HIV Replication and Selects the Reverse Transcriptase V75I Multi-Drug Resistance Mutation. J Biol Chem. 2008, 283: 31289-31293. (PMID 18818198)
Kohli RM, Walsh CT. Enzymology of acyl chain macrocyclization in natural product biosynthesis. Chem Commun 2003:297-307. (PMID 12613585)
Yeh E, Lin H, Clugston SL, Kohli RM, Walsh CT. Enhanced macrocyclizing activity of the thioesterase from tyrocidine synthetase in presence of nonionic detergent. Chem Biol. 2004, 11:1573-1582. (PMID 15556008)
Yeh E, Kohli RM, Bruner SD, Walsh CT. Type II thioesterase restores activity of a NRPS module stalled withan aminoacyl-S-enzyme that cannot be elongated. ChemBiochem. 2004: 1290-1293. (PMID 15368584)
Kohli RM, Burke MD, Tao J, Walsh CT. Chemoenzymatic route to hybrid peptide/polyketide-like molecules. J Am Chem Soc 2003, 125:7160-7161. (PMID 12797773)
Tseng CC, Bruner SD, Kohli RM, Marahiel MA, Walsh CT, Sieber SA. Characterization of the surfactin synthetase C-terminal thioesterase domain as a cyclic depsipeptide synthase. Biochemistry 2002, 41:13350-13359. (PMID 12416979)
Kohli RM, Walsh CT, Burkart MD. Biomimetic synthesis and optimization of cyclic peptide antibiotics. Nature 2002, 418:658-661. (PMID 12167866)
[Comment in Nature Drug Discov 2002, 1: 748; C & E News 2002, 80: 10]
Luo L, Kohli RM, Onishi M, Linne U, Marahiel MA, Walsh CT. Timing of epimerization and condensation reactions in nonribosomal peptide assembly lines: kinetic analysis of phenylalanine activating elongation modules of tyrocidine synthetase B. Biochemistry 2002, 41: 9184-9196. (PMID 12119033)
Bruner SD, Weber T, Kohli RM, Schwarzer D, Marahiel MA, Walsh CT, Stubbs MT. Structural basis for the cyclization of the lipopeptide antibiotic surfactin by the thioesterase domain SrfTE. Structure 2002, 10: 301-310. (PMID 12005429)
Kohli RM, Takagi J, Walsh CT. The thioesterase domain from a nonribosomal peptide synthetase as a cyclization catalyst for integrin binding peptides. Proc Natl Acad Sci U S A 2002, 99:1247-1252. (PMID 11805307)
[Comment in TRENDS Biochem Sci 2002, 27: 119-120]
Keating TA, Ehman DE, Kohli RM, Marshall CG, Trauger JW, Walsh CT. Chain termination steps in nonribosomal peptide synthetase assembly lines: Directed acyl-S-enzyme breakdown in antibiotic and siderophore biosynthesis. ChemBiochem 2001, 2: 99-107. (PMID 11828432)
Trauger JW, Kohli RM, Walsh CT. Cyclization of backbone-substituted peptides by the thioesterase domain from the tyrocidine nonribosomal peptide synthetase. Biochemistry 2001, 40: 7092-7098. (PMID 11401554)
Kohli RM, Trauger JW, Schwarzer D, Marahiel MA, Walsh CT. Generality of peptide cyclization catalyzed by isolated thioesterase domains of nonribosomal peptide synthetases. Biochemistry 2001, 40: 7099-7108. (PMID 11401555)
[Comment in Curr Opin Chem Biol 2001, 5: 471]
Trauger JW, Kohli RM, Mootz HD, Marahiel MA, Walsh CT. Peptide cyclization catalyzed by the thioesterase domain of tyrocidine synthetase. Nature 2000, 407: 215-218. (PMID 11001063)
Xu D, Kohli RM, Massey V. The role of threonine 37 in flavin reactivity of the Old Yellow Enzyme. Proc Natl Acad Sci USA 1999, 96: 3556-3561. (PMID 10097075)
Kohli RM, Massey V. The oxidative half-reaction of Old Yellow Enzyme. J Biol Chem 1998, 273: 32763-32770. (PMID 9830020)
Kohli RM. Site-directed mutagenesis using PCR-mediated introduction of silent mutations. Biotechniques 1998, 25: 184-188. (PMID 9714873)
• Last updated: 11/09/2012