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Vitamin D-Binding Protein (DBP) Project

  • Defining the Pathway of hDPB Locus Activation in Hepatic Chromatin
  • Assigning Novel Physiologic Functions to DBP
Project Summary
Vitamin D-Binding Protein (DBP) Gene Expression;
Defining the Pathway of hDPB Locus Activation in Hepatic Chromatin and Assigning Novel Physiologic Functions to DBP
The DBP group studies Vitamin D-binding protein (DBP, also known as the group-specific component of serum: GC-globulin). This is a highly expressed serum protein that is a member of the albumin (ALB), ±-fetoprotein (AFP), and afamin or ±-albumin (AFM) gene family. These four genes are linked on human chromosome 4. DBP is a highly polymorphic serum protein predominantly synthesized in liver as a single-chain glycoprotein of approximately 58 kDa. Studies include: the function of DBP via gene knock-out and transgenic approaches in mice, and the basis for its robust and liver-specific transcriptional activation via defined chromatin determinants.

Project Personnel

      Tomoko Hiroki, Ph.D.
  Chris Laing, Ph.D.
Selected References
Primary Papers (1995 - present)
  1. Tang, W. X., Bazaraa, H.M., Magiera, H., Cooke, N.E., Haddad, J.G.: Electrophoretic Mobility Shift Assay Identifies Vitamin D Binding Protein (Gc-globulin) in Human, Rat, and Mouse Sera. Anal. Biochem. 237: 245 - 251, 1996.

  2. Song Y-H, Ray K, Liebhaber SA, Cooke NE. Vitamin D binding protein gene transcription is regulated by the relative abundance of HNF-1? and HNF-1?. J. Biol. Chem. 273:28408-28418, 1998.

  3. Safadi F, Thornton P, Magiera HM, Hollis B, Gentile M, Haddad JG,Liebhaber SA, Cooke NE. Osteopathy and resistance to vitamin D toxicity in mice null for vitamin D binding protein. J. Clin. Invest 103:239-251, 1999.

  4. Song Y-H, Naumova A, Liebhaber SA, Cooke NE. Physical and Meiotic Mapping of the region of human chromosome 4q11-13 encompassing the vitamin D binding protein (DBP/Gc-globulin) and albumin multigene cluster. Genome Research 9:581-587, 1999.

  5. White, P. Liebhaber, SA, and Cooke, NE. 2002 The 129X1/SvJ mouse strain has a novel defect in inflammatory cell recruitment. J. Immunology. 168:869-874, 2002.

  6. Hiroki, T., Song Y-H., Liebhaber, S.A., and Cooke, N.E. The hDBP gene contains chromatin determinants sufficient for autonomous gene activation. Submitted.

  7. Hiroki T, Song Y-H, Liebhaber S A, and Cooke N E.  2006. The Human Vitamin D-binding Protein Gene Contains Locus Control Determinants Sufficient for Autonomous Activation in Hepatic Chromatin. Nucleic Acids Res 34: 2154-2165, 2006. Download PDF

Reviews (1995 - present)

  1. Cooke, N.E. and Haddad, J.G.: Chapter 7: "Vitamin D-Binding Protein", in Feldman, D., Glorieux, F., Pike, JW., eds. Vitamin D, Academic Press, San Diego, CA, 1997, pp. 87-101.

  2. Haddad JG and Cooke NE. "Plasma vitamin D binding protein/Gc-globulin" in Kreis T and Vale R (eds), Guidebook to the Cytoskeleton and Motor Proteins, Oxford University Press, 2nd ed., 1999, pp. 179-181.

  3. White P, Cooke NE. The multifunctional properies and characteristics of vitamin D binding protein (DBP). Trends Endocrinol Metab 11:320-327, 2000.

  4. Lang, C. and Cooke, NE. "Vitamin D Binding Protein;. In Vitamin D. second edition. Eds; Feldman, Pike, and Glorieux . Elsivier Press. In Press 2004
Last Updated: Tue, Aug 31, 2004