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Mouse b-Actin primers
primers correspond to gi|49865|emb|X03672.1|MMACTBR Mouse cytoskeletal mRNA for beta-actin, F is base pair 33-56 and R is 490' to 467' yielding a 457 base pair PCR product.
Beta Actin F primer
TTC TTT GCA GCT CCT TCG TTG CCG
Beta Actin R primer
TGGATGGCTACGTACATGGCTGGG
PCR cocktail : for each 100 l solutionÂ
5l Beta Actin F primer, 10 mM stock
5l Beta Actin R primer, 10 mM stock
10l 10X PCR buffer
2l dNTP 10 mM stock
1l Taq polymerase
77l H2OÂ
PCR conditions : 94ËsC 1 minute
94ËsC 1 minute
60ËsC 1 minute repeat for 32 cycles
72ËsC 1 minute
72ËsC 5 minute
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