• Jon Epstein,
  MCRC Director
• Michael Parmacek,
  Penn CVI Director
• Ed Morrisey
• Mark L. Kahn
• Peter Gruber
• Vic Patel
• Ken Margulies
• Cardiac Myocyte Core
• Histology and
  Gene Expression Core
• Microscopy Core
• Physiology and
  Microsurgery Core
• Plasmid Core
• Transgenic and
  Knockout Core
• Information for Staff
• Useful Links
• Search MCRC
• Penn CVI
• Penn CVI Seminars

PCR Strategy Pax3Promoter-Cre Transgenic Mice

Reagents

1. Primers (100 µM in H₂0)

Cre-F-BSTE11 5'-AAT CTT ATG GTC ACC TGA GTG TTA AAT GTC CAA

TTT AC

Cre-R. 5'-CAT CTT CAG GTT CTG CGG G

PCR Product.230 bp

2. Example Reaction

10 x PCR Buffer 10λ

25mM dNTP's 0.8λ

primer Cre-F-BSTE11 0.5λ

Cre-R 0.5λ

Taq Polmerase 0.5λ

H2 O 87.7λ

Total 100λ

Aliquot 30λ to each tube. Add 1.0λ DNA to the reaction tube.

3. Program

94°C 1 Min

60°C 1 Min

72°C 1 Min

go to step 1 repeat 35 cycles

72°C 5 Min

4°C 99hrs

» Top