Histology and Gene Expression Core
Description
This site contains documents, protocols and suggestions relevant to the procedures performed in the Histology and Gene Expression Core Facility. The Core provides expert professional services for members of the Cardiovascular Division. Services for non-division investigators is available on a fee-for-service basis as time permits.
Please contact Dr. Min Min Lu at mlu2@mail.med.upenn.edu to inquire further about these services.
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Contact
Dr. Min Min Lu - Director
BRB II/III Room 947
421 Curie Blvd
University of Pennsylvania
Philadelphia, PA 19104-6160
Email: mlu2@mail.med.upenn.edu
Phone: 215-898-0251
Lan Cheng, lancheng@mail.med.upenn.edu, 215-573-2328
Jifu Yang, jifu@mail.med.upenn.edu, 215-573-2327
Jisheng Yang, jisheng@mail.med.upenn.edu, 215-573-2328
Li Li, li10@mail.med.upenn.edu, 215-573-2327
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Forms and Instructions
Instructions for Submitting Samples
- Submit Samples for Processing and Parafin Sectioning [doc]
Submit Samples for Frozen Sectioning [doc]
- Submit Embedded Blocks for Sectioning [doc]
- Submit Slides for HE or Eosin Staining [doc]
- Submit Paraffin Slides for Immunohistochemistry (IHC) Staining [doc]
Submit Frozen Slides for Immunohistochemistry (IHC) Staining [doc]
Submit IHC Form [doc]
- Submit Slides for Other Staining [doc]
- Submit Sections for In Situ Hybridization [doc]
- # of Sets and # of Slides [pdf]
Histology Request Forms
- Submission of Tissue Form [doc]
- Project Form [FileMaker Pro 7]
- In Situs Form [FileMaker Pro 7]
- IHC Form [FileMaker Pro 7]
- Whole Mount Immuno and In Situs [doc]
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References ~
- Antibody List [FileMaker Pro 7]
- Template List [FileMaker Pro]
- Mouse Embryos Section Reference Table [FileMaker Pro 7]
Protocols
Histology Protocols
- How to Make 4% PFA
- How to Fix and Dehydrate Tissue [doc]
- How to Perform H&E Staining [doc]
- Decalcification Using EDTA
- McMannus' Periodic Acid Schiff's (PAS) Stain
- Verhoeff-Van Gieson (Elastin) Stain
- Masson's Trichrome Stain
- Modified Movat's Pentachrome Stain
Immunostaining Protocols
General Protocols ~
- How to Prepare Tissue for IHC in Paraffin Section [doc]
- How to Prepare Tissue for IHC in Frozen Sections
- How to Prepare Tissue for Whole Mount IHC [doc]
- How to Prepare Cultured Cells for IHC
- Protein A Purification of Polyclonal Ab
- ABC-AP
- ABC-HRP [doc]
- TSA-FITC [doc]
- Antigen Unmasking/Retrieval
- Immunofloresence on Paraffin Sections [doc]
- Immunofloresence on Frozen Sections [doc]
Individual Protocols ~
- Rat Anit-PECAM in Paraffin Embedded Sections (TSA)
Rabbit Anti β-gal in Paraffin Embedded Sections (Alexa Fluo 488)
Goat Anti GFP in Paraffin Embedded Sections (Alexa Fluo 488)
Mouse anti smooth muscle α-actin in Paraffin Embedded Sections (Alexa Fluo 568)
Rabbit Anti-P-ERK in Paraffin Embedded Sections (ABC HRP)
Apoptosis and Proliferation Protocols
In Situ Hybridization Protocols
- How to Prepare a DNA Template for Riboprobe Synthesis
- How to Make a Radioactive Riboprobe
- How to Prepare Tissue for In Situs
- How to Process Embryos for Whole Mount In Situs
- Radioactive In Situs Protocol
- Non-radioactive In Situs Protocol
- Whole Mount In Situs Protocol
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