Preparation of Cultured Cell for Immunostaining
- Wash cells with PBS 1min X2.
- Fix with 4% PF for 10 min.
- Wash in PBST (PBS+0.1% Tween-20)2min X3.
- Dehydrate with Methanol and PBST
25% Methanol in PBT 5min.
50% Methanol in PBT 5min.
75 % Methanol in PBT 5min.
100 % Methanol 5min.
- Change to 100 % Methanol and keep at -20°C.