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Preparation of Cultured Cell for Immunostaining

1. Wash cells with PBS 1min X2.
2. Fix with 4% PF for 10 min.
3. Wash in PBST (PBS+0.1% Tween-20)2min X3.
4. Dehydrate with Methanol and PBST
   25% Methanol in PBT 5min.
   50% Methanol in PBT 5min.
   75 % Methanol in PBT 5min.
   100 % Methanol 5min.
5. Change to 100 % Methanol and keep at -20°C.

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