Histology and Gene Expression Core
Description
This site contains documents, protocols and suggestions relevant to the procedures performed in the Histology and Gene Expression Core Facility. The Core provides expert professional services for members of the Cardiovascular Division. Services for non-division investigators is available on a fee-for-service basis as time permits.
Please contact Dr. Min Min Lu at mlu2@mail.med.upenn.edu to inquire further about these services.
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Contact
Dr. Min Min Lu - Director
BRB II/III Room 1049
421 Curie Blvd
University of Pennsylvania
Philadelphia, PA 19104-6160
Email: mlu2@mail.med.upenn.edu
Phone: 215-898-0251
Lan Cheng, lancheng@mail.med.upenn.edu, 215-573-2328
Jifu Yang, jifu@mail.med.upenn.edu, 215-573-2328
Jisheng Yang, jisheng@mail.med.upenn.edu, 215-573-2328
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Forms and Instructions
Instructions for Submitting Samples
- Submit Samples for Processing and Sectioning [doc]
- Submit Embedded Blocks for Sectioning [doc]
- Submit Slides for HE or Eosin Staining [doc]
- Submit Sections for Immunohistochemistry (IHC) Staining [doc]
- Submit Slides for Other Staining [doc]
- Submit Sections for In Situ Hybridization [doc]
- # of Sets and # of Slides [pdf]
- Section Orientation and Interested Area [pdf]
Histology Request Forms
- Submission of Tissue Form [doc]
- Project Form [FileMaker Pro 7]
- In Situs Form [FileMaker Pro 7]
- IHC Form [FileMaker Pro 7]
- Whole Mount Immuno and In Situs [doc]
Histology Protocols
- How to Make 4% PFA
- How to Fix and Dehydrate Tissue [doc]
- How to Perform H&E Staining [doc]
- Decalcification Using EDTA
- McMannus' Periodic Acid Schiff's (PAS) Stain
- Verhoeff-Van Gieson (Elastin) Stain
- Masson's Trichrome Stain
- Modified Movat's Pentachrome Stain
Immunostaining Protocols
- How to Prepare Tissue for Paraffin Section
- How to Prepare Tissue for Frozen Sections
- How to Prepare Tissue for Whole Mount [doc]
- How to Prepare Cultured Cells
- Protein A Purification of Polyclonal Ab
- ABC-AP
- ABC-HRP
- Antigen Unmasking/Retrieval
- Rabbit Anti β-gal Using Paraffin Embedded Sections
- Mouse Anti β-gal Using Paraffin Embedded Sections
- Immunofluorescence protocol: FITC, or Texas Red
- Rat Anit-PECAM in Paraffin
- Rabbit Anti-P-ERK in Paraffin Embedded Sections
- Indirect FITC or Texas Red on Frozen Sections
Apoptosis and Proliferation Protocols
In Situ Hybridization Protocols
- How to Prepare a DNA Template for Riboprobe Synthesis
- How to Make a Radioactive Riboprobe
- How to Prepare Tissue for In Situs
- How to Process Embryos for Whole Mount In Situs
- Radioactive In Situs Protocol
- Non-radioactive In Situs Protocol
- Whole Mount In Situs Protocol
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